3D multiplex visualization of biologic distribution in intact tumors and organs using light-sheet microscopy

Niyanta Kumar

doi:10.1117/12.2610685

7 March 2022 3D multiplex visualization of biologic distribution in intact tumors and organs using light-sheet microscopy

Niyanta Kumar

Author Affiliations +

Proceedings Volume PC11938, Visualizing and Quantifying Drug Distribution in Tissue VI; PC119380B (2022) https://doi.org/10.1117/12.2610685
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Ex vivo distribution studies of macromolecular drugs, targets, and biomarkers have been typically limited to bulk sampling methods requiring tissue homogenization or 2D imaging. Bulk sampling cannot capture the complex spatial heterogeneity in tumors and 2D imaging techniques limit analysis to a few representative tissue sections. In this work we explored a tissue clearing, immunolabeling, and lightsheet imaging methods to visualize the ex vivo distribution of biologics in a preclinical models. Biologic distribution in intact tumors was assessed at sub-cellular resolution. A data handling and analysis workflow was developed to assess biologic penetration in tumors and influence of vascular heterogeneity. This work demonstrates potential applications to examine biologic drug distribution-efficacy relationships for drug discovery and development.

Conference Presentation

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Niyanta Kumar "3D multiplex visualization of biologic distribution in intact tumors and organs using light-sheet microscopy", Proc. SPIE PC11938, Visualizing and Quantifying Drug Distribution in Tissue VI, PC119380B (7 March 2022); https://doi.org/10.1117/12.2610685

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