Fourier-transform Fluorescence Recovery after Photobleaching (FT-FRAP) diffusion imaging analysis

Ziyi Cao; Dustin Harmon; Jiayue Rong; Andreas Geiger; Garth J. Simpson

doi:10.1117/12.2607631

2 March 2022 Fourier-transform Fluorescence Recovery after Photobleaching (FT-FRAP) diffusion imaging analysis

Ziyi Cao, Dustin Harmon, Jiayue Rong, Andreas Geiger, Garth J. Simpson

Author Affiliations +

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730J (2022) https://doi.org/10.1117/12.2607631
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Periodically patterned photobleaching followed by spatial Fourier transform analysis of the recovery is shown to enable mapping of molecular diffusivity within spatially heterogeneous media. Both pixel by pixel analysis method and segmented analysis method of Fourier transform fluorescence recovery after photobleaching (FT-FRAP) measurements and implemented for quantitatively evaluating diffusion of macromolecules in spatially and chemically complex media. With the development of FT-FRAP, the time-dependent recovery in fluorescence due to diffusion is measured in the spatial Fourier domain, with substantial improvements in the signal-to-noise ratio, the mathematical simplicity, and the compatibility with multi-photon excitation.

Conference Presentation

Citation Download Citation

Ziyi Cao, Dustin Harmon, Jiayue Rong, Andreas Geiger, and Garth J. Simpson "Fourier-transform Fluorescence Recovery after Photobleaching (FT-FRAP) diffusion imaging analysis", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730J (2 March 2022); https://doi.org/10.1117/12.2607631

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