UV-visible-NIR self-phase-modulation-enabled femtosecond fiber source for biomedical single/two-photon fluorescence microscopy

Zi-Ping Chen; Lu-Ting Chou; Yu-Cheng Liu; Shih-Hsuan Chia

doi:10.1117/12.2609799

1 April 2022 UV-visible-NIR self-phase-modulation-enabled femtosecond fiber source for biomedical single/two-photon fluorescence microscopy

Zi-Ping Chen, Lu-Ting Chou, Yu-Cheng Liu, Shih-Hsuan Chia

Author Affiliations +

Proceedings Volume PC11991, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XXII; PC1199105 (2022) https://doi.org/10.1117/12.2609799
Event: SPIE LASE, 2022, San Francisco, California, United States

Abstract

We optimized the spectral coverage of self-phase-modulation-enabled femtosecond fiber sources by careful investigations into the influence of input pulse width, fiber length, and fiber damage, and we have demonstrated a widely tunable source ranging from 740-1250 nm for two-photon microscopy applications. In addition, tens of milliwatt tunable near-UV/visible spectrum is easily obtained by a frequency-doubled conversion, and the gap between the fundamental and frequency-doubled spectra can be filled with the nonlinear wave breaking around 650 nm. A multi-modality microscopy incorporating two-photon microscopy and confocal fluorescence microscopy was also demonstrated to prove the versatility of our development for biomedical imaging.

Conference Presentation

Citation Download Citation

Zi-Ping Chen, Lu-Ting Chou, Yu-Cheng Liu, and Shih-Hsuan Chia "UV-visible-NIR self-phase-modulation-enabled femtosecond fiber source for biomedical single/two-photon fluorescence microscopy", Proc. SPIE PC11991, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XXII, PC1199105 (1 April 2022); https://doi.org/10.1117/12.2609799

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