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Impulsive stimulated Brillouin microscopy (ISBS) is a technique that promises to enable fast measurements of the viscoelastic properties of biological samples. Its big advantage compared to spontaneous Brillouin arises from the capability to manipulate the signal-to-noise ratio by the choice of the system parameters. In this contribution, we will present a thorough analysis of the signal generation dependencies and the resulting implications on the spatiotemporal resolution. First measurements on blood, water and hydrogels underline the potential of the technique and show that ISBS can be an important alternative for selected biomedical applications.
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Nektarios Koukourakis, Leon Liebig, Jürgen W. Czarske, "Impulsive stimulated Brillouin microscopy for high-speed biomedical diagnostics (Conference Presentation)," Proc. SPIE PC12381, Optical Elastography and Tissue Biomechanics X, PC1238109 (15 March 2023); https://doi.org/10.1117/12.2651776