Toward high throughput 3D connectome imaging (Conference Presentation)

Fu-Jen Kao; Ann-Shyn Chiang; Scotte Liu; Tiffany Kao; Daniel Wu; Wei-Kun Chang; Henry Liu; Jerry Wong

doi:10.1117/12.2654741

15 March 2023 Toward high throughput 3D connectome imaging (Conference Presentation)

Fu-Jen Kao, Ann-Shyn Chiang, Scotte Liu, Tiffany Kao, Daniel Wu, Wei-Kun Chang, Henry Liu, Jerry Wong

Author Affiliations +

Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC123840M (2023) https://doi.org/10.1117/12.2654741
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We will present the initiative in imaging a hugely expanded drosophila brain via expansion microscopy, which has brought unprecedented opportunities and challenges. 100X expansion has been demonstrated in enlarging the typically 0.6x0.3x0.2 mm brain to 60x30x20 mm, which promises electron microscopy resolution (approximately 3 nm) with optical microscopy (approximately 300 nm) to resolve the synapses connection. A highly sped-up imaging method integrated with an ultrasound-activated microtome is crucial to support the initiative. Critically, photon statistics set the fundamental considerations in selecting the contrasts for optical imaging.

Conference Presentation

Citation Download Citation

Fu-Jen Kao, Ann-Shyn Chiang, Scotte Liu, Tiffany Kao, Daniel Wu, Wei-Kun Chang, Henry Liu, and Jerry Wong "Toward high throughput 3D connectome imaging (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840M (15 March 2023); https://doi.org/10.1117/12.2654741

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