Measurement of the optical properties of fluorescent media using an integrating sphere

Thomas Brall; Florian Foschum; Florian Bergmann; Alwin Kienle

doi:10.1117/12.2673255

10 August 2023 Measurement of the optical properties of fluorescent media using an integrating sphere

Thomas Brall, Florian Foschum, Florian Bergmann, Alwin Kienle

Author Affiliations +

Proceedings Volume PC12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; PC126220W (2023) https://doi.org/10.1117/12.2673255
Event: SPIE Optical Metrology, 2023, Munich, Germany

Conference Poster

Abstract

To measure optical properties of turbid fluorescent samples, an advancement of an inhouse developed integration sphere system was build up. This system based on a 3D printed integrating sphere uses photodiodes with lock-in technics for detection. The tuneable monochromatic illumination is realized by a laser pumped xenon light source with an integrated monochromator. A second monochromator on the detection side allows to discriminate between inelastically and elastically scattered light from the fluorescent media. By measuring the reflection and transmission from the investigated turbid sample, the wavelength dependent optical properties (absorption coefficient µa, effective scattering coefficient µs’) as well as the quantum efficiency (or concentration) of the fluorophore can be determinate using lookup tables generated from Monte Carlo simulations. Validation measurements are performed on turbid rhodamine phantoms.

Citation Download Citation

Thomas Brall, Florian Foschum, Florian Bergmann, and Alwin Kienle "Measurement of the optical properties of fluorescent media using an integrating sphere", Proc. SPIE PC12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, PC126220W (10 August 2023); https://doi.org/10.1117/12.2673255

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