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6 October 2023 Label-free imaging of cellular activity through second-harmonic generation
Yovan de Coene, Carmen Bartic, Koen Clays, Thierry Verbiest, Stijn Jooken, Stijn van Cleuvenbergen, Pieter Vanden Berghe
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Proceedings Volume PC12681, Ultrafast Nonlinear Imaging and Spectroscopy XI; PC1268105 (2023) https://doi.org/10.1117/12.2676376
Event: SPIE Optical Engineering + Applications, 2023, San Diego, California, United States
Abstract
Optical interrogation of cellular electrical activity is a crucial tool for understanding how cells function and communicate in complex networks. Scientists often use voltage-sensitive dyes to measure the excitability of cells, but these dyes can interfere with cellular function. Label-free techniques offer a way to measure electrical activity without using external probes. In this study, researchers found that second-harmonic generation from live cells is highly sensitive to changes in transmembrane potential by electrode control, making it a promising label-free approach to measure electrical activity in more complex cellular networks. This research provides a promising framework for a non-invasive label-free tool to measure electrical activity in cells.
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Yovan de Coene, Carmen Bartic, Koen Clays, Thierry Verbiest, Stijn Jooken, Stijn van Cleuvenbergen, and Pieter Vanden Berghe "Label-free imaging of cellular activity through second-harmonic generation", Proc. SPIE PC12681, Ultrafast Nonlinear Imaging and Spectroscopy XI, PC1268105 (6 October 2023); https://doi.org/10.1117/12.2676376
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KEYWORDS
Second harmonic generation
Biological imaging
Dyes
Electric fields
Electrolytes
Kidney
Optical surfaces
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