Combining photoacoustic microscopy and fluorescence microscopy imaging for evaluating Alzheimer's disease progression in mouse model

Tianqu Zhai; Wei Zhang; Yannis M. Paulus; Enming J. Su; Geoffrey Murphy; Daniel A. Lawrence; Xueding Wang

doi:10.1117/12.3002807

13 March 2024 Combining photoacoustic microscopy and fluorescence microscopy imaging for evaluating Alzheimer's disease progression in mouse model

Tianqu Zhai, Wei Zhang, Yannis M. Paulus, Enming J. Su, Geoffrey Murphy, Daniel A. Lawrence, Xueding Wang

Author Affiliations +

Proceedings Volume PC12828, Neural Imaging and Sensing 2024; PC128280A (2024) https://doi.org/10.1117/12.3002807
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Alzheimer’s disease (AD) is a neurodegenerative disorder usually accompanied by buildup of amyloid-beta (Aβ) plaques and neurofibrillary tangles, cerebral vascular morphological and functional changes. Our study presents a combined photoacoustic microscopy and fluorescence microscopy dual-modality imaging system for in-vivo monitoring of biomarkers for AD via cranial window. Photoacoustic microscopy resolves vasculature, blood oxygenation and flow speed at capillary level, while a confocally designed fluorescence microscope, aided by an Aβ targeting dye, maps the distribution of Aβ plaques. Results with age-varied AD mice group showed reduced capillary density, varied flow speed in vein, and increased plaque density compared to wild type mice. This technique provides guidance for longitudinal monitoring of AD progression.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Tianqu Zhai, Wei Zhang, Yannis M. Paulus, Enming J. Su, Geoffrey Murphy, Daniel A. Lawrence, and Xueding Wang "Combining photoacoustic microscopy and fluorescence microscopy imaging for evaluating Alzheimer's disease progression in mouse model", Proc. SPIE PC12828, Neural Imaging and Sensing 2024, PC128280A (13 March 2024); https://doi.org/10.1117/12.3002807

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