Synthetic aperture third harmonic generation holographic microscopy

Yusef R. Farah; Gabe Murray; Jeff Field; Lang Wang; Maxine Xiu; Olivier Pinaud; Randy Bartels

doi:10.1117/12.3003487

13 March 2024 Synthetic aperture third harmonic generation holographic microscopy

Yusef R. Farah, Gabe Murray, Jeff Field, Lang Wang, Maxine Xiu, Olivier Pinaud, Randy Bartels

Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC128470N (2024) https://doi.org/10.1117/12.3003487
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Imaging with third harmonic generation (THG) provides a powerful label-free approach to probing biological systems, particularly looking at structures formed by lipids. We present the first demonstration of THG widefield holographic microscopy. Holographic microscopy preserves the amplitude and phase of the measured field, which enables us to construct a synthetic spatial aperture with expanded field of view for complex valued THG images. Additionally, we implement a matrix approach postprocessing algorithm to reconstruct aberration-free THG holographic images by estimating and correcting for phase aberrations and scattering distortions.

Conference Presentation

Citation Download Citation

Yusef R. Farah, Gabe Murray, Jeff Field, Lang Wang, Maxine Xiu, Olivier Pinaud, and Randy Bartels "Synthetic aperture third harmonic generation holographic microscopy", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470N (13 March 2024); https://doi.org/10.1117/12.3003487

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available