Towards measurements of blood hematocrit with in-vivo retinal imaging

Vincent S. Zoutenbier; Peter Bussink; Arjen Amelink

doi:10.1117/12.3001031

13 March 2024 Towards measurements of blood hematocrit with in-vivo retinal imaging

Vincent S. Zoutenbier, Peter Bussink, Arjen Amelink

Proceedings Volume PC12850, Optical Diagnostics and Sensing XXIV: Toward Point-of-Care Diagnostics; PC128500G (2024) https://doi.org/10.1117/12.3001031
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

The accurate measurement of blood hematocrit levels in ocular vessels holds significant clinical value by saving time, minimizing discomfort, and reducing the use of single-use cuvettes, which generates significant biohazardous waste. We present a novel approach for non-invasive measurement of blood hematocrit levels in retinal vasculature using a modified full-field fundus imaging system centered around two isosbestic wavelengths. To aid in the selection of bandpass filters, we developed a detailed Monte-Carlo simulation of our imaging system applied to retinal vasculature. We also present this model as a tool to analyze the images coming from the Fundus camera, which helps us understand what tissue and system parameters affect our in-vivo measurements. We present the analysis methods employed for data from a clinical trial data and demonstrate the trends between the optical densities of the collected images and our Monte Carlo simulations.

Citation Download Citation

Vincent S. Zoutenbier, Peter Bussink, and Arjen Amelink "Towards measurements of blood hematocrit with in-vivo retinal imaging", Proc. SPIE PC12850, Optical Diagnostics and Sensing XXIV: Toward Point-of-Care Diagnostics, PC128500G (13 March 2024); https://doi.org/10.1117/12.3001031

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