Two-photon microscopy with ultrafast scattering control for deep tissue imaging

Yi Xue

doi:10.1117/12.3003625

13 March 2024 Two-photon microscopy with ultrafast scattering control for deep tissue imaging

Yi Xue

Author Affiliations +

Proceedings Volume PC12851, Adaptive Optics and Wavefront Control for Biological Systems X; PC128510N (2024) https://doi.org/10.1117/12.3003625
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Deep brain imaging at cellular resolution is challenging due to strong and dynamic tissue scattering. Previous techniques for scattering correction are either slow or not in reflection mode, hindering them from imaging deep in the mouse brain in vivo. Here, we have developed a new two-photon microscope with ultrafast scattering correction for deep brain imaging. We use a compressive sensing algorithm to calculate correction masks from much fewer measurements than previous methods and couple light into high-transmission channels of scattering tissue using a digital micromirror device. We demonstrate real-time correction of dynamic scattering by focusing through various dynamic scattering samples.

Conference Presentation

Citation Download Citation

Yi Xue "Two-photon microscopy with ultrafast scattering control for deep tissue imaging", Proc. SPIE PC12851, Adaptive Optics and Wavefront Control for Biological Systems X, PC128510N (13 March 2024); https://doi.org/10.1117/12.3003625

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