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13 March 2024 Quantitative phase imaging of intracellular liquids
Yongdae Shin
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Proceedings Volume PC12852, Quantitative Phase Imaging X; PC128520J (2024) https://doi.org/10.1117/12.3000437
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
The cell interior is highly compartmentalized into various biomolecular condensates. The biophysical properties of condensates are thought to be tightly linked to their functions, yet a deeper understanding of this relationship is hampered by a lack of tools to quantitatively probe the organization of condensates in living cells. Here, we use optical diffraction tomography (ODT) to measure the biomolecular density of condensates. We find that intracellular condensates exhibit a broad range of biomolecular densities. The low-density ones exhibit densities similar to the surrounding cyto- or nucleoplasm, and are highly permeable to cellular protein probes. We also uncover that RNA plays a key role in tuning the density of condensates. Our work highlight that ODT is a powerful tool for exploring the complexity of the intracellular phases.
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Yongdae Shin "Quantitative phase imaging of intracellular liquids", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC128520J (13 March 2024); https://doi.org/10.1117/12.3000437
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KEYWORDS
Liquids
Phase imaging
Biomolecules
Mitochondria
Molecular interactions
Permeability
Proteins
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