Rapid quantitative phase imaging with acousto-optic focus control and light engineering

Christos Alexandropoulos; Marti Duocastella

doi:10.1117/12.3002885

13 March 2024 Rapid quantitative phase imaging with acousto-optic focus control and light engineering

Christos Alexandropoulos, Marti Duocastella

Proceedings Volume PC12852, Quantitative Phase Imaging X; PC128521J (2024) https://doi.org/10.1117/12.3002885
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

The transport of intensity (TIE) technique allows for label-free quantitative phase imaging by acquiring two or more images from different focal planes. However, current TIE systems can be limited in speed or accuracy by methods used for the control and selection of the focal planes. Here, we introduce a novel microscope that combines an acousto-optic liquid lens (TAG lens) with illumination engineering to achieve precise electronic defocus control, indistinguishable from traditional mechanical defocusing, at microsecond timescales. Such high speed enables phase-imaging of dynamic events, including an evaporating droplet or a moving microorganism, at diffraction-limited resolution and rates of up to 150 Hz.

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Christos Alexandropoulos and Marti Duocastella "Rapid quantitative phase imaging with acousto-optic focus control and light engineering", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC128521J (13 March 2024); https://doi.org/10.1117/12.3002885

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