Time delay integration Raman flow cytometry

Matthew Lindley; Toshiki Kubo; Stéphanie Devineau; Menglu Li; Katsumasa Fujita

doi:10.1117/12.3003149

13 March 2024 Time delay integration Raman flow cytometry

Matthew Lindley, Toshiki Kubo, Stéphanie Devineau, Menglu Li, Katsumasa Fujita

Proceedings Volume PC12853, High-Speed Biomedical Imaging and Spectroscopy IX; PC128530K (2024) https://doi.org/10.1117/12.3003149
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Spontaneous Raman flow cytometers and cell sorters typically have cell or particle throughputs less than 5 event/s due to long signal integration times. Here we report an up to 10x increase in throughput by coupling constant illumination along the flow path with time delay integration, a technique that counteracts sample motion by matching it to the charge transfer velocity of a CCD device. This allows longer signal integration times while also multiplexing acquisition along the flow path. We demonstrate high spectral bandwidth (600 – 3200 cm-1) Raman acquisitions from flowing particles and mammalian cells at throughputs up to 50 events/s.

Conference Presentation

Citation Download Citation

Matthew Lindley, Toshiki Kubo, Stéphanie Devineau, Menglu Li, and Katsumasa Fujita "Time delay integration Raman flow cytometry", Proc. SPIE PC12853, High-Speed Biomedical Imaging and Spectroscopy IX, PC128530K (13 March 2024); https://doi.org/10.1117/12.3003149

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