High-speed simultaneous label-free autofluorescence-multiharmonic (hSLAM) microscopy for multicellular dynamics

Kunzan Liu; Tong Qiu; Honghao Cao; Linda G. Griffith; Fan Wang; Sixian You

doi:10.1117/12.3002856

13 March 2024 High-speed simultaneous label-free autofluorescence-multiharmonic (hSLAM) microscopy for multicellular dynamics

Kunzan Liu, Tong Qiu, Honghao Cao, Linda G. Griffith, Fan Wang, Sixian You

Author Affiliations +

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC1285406 (2024) https://doi.org/10.1117/12.3002856
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Label-free nonlinear microscopy allows for high-resolution and three-dimensional imaging of live biological specimens without the need for exogenous labels. The integration of multiple modalities further enhances molecular specificity and visualization diversity for metabolic and structural mapping of heterogeneous tissue architectures. In this work, we introduce high-speed simultaneous label-free autofluorescence-multiharmonic (hSLAM) microscopy, where a high-peak-power adaptive fiber source based on multimode fiber (MMF) is employed with a nonlinear fiber piano. We will also talk about how the higher speed SLAM enables multicellular dynamics in living tissues with higher spectral flexibility and peak power, providing new possibilities for bioimaging.

Conference Presentation

Citation Download Citation

Kunzan Liu, Tong Qiu, Honghao Cao, Linda G. Griffith, Fan Wang, and Sixian You "High-speed simultaneous label-free autofluorescence-multiharmonic (hSLAM) microscopy for multicellular dynamics", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC1285406 (13 March 2024); https://doi.org/10.1117/12.3002856

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