Macroscale fluorescence lifetime imaging with novel time-gated camera complemented with high-resolution color image registration

Sevada Sahakian; Simone Janssen; Thomas Van den Dries; Thomas Lapauw; Marcus Stroet; Théo Lepoutte; Pooria Iranian; Maarten Kuijk; Sophie Hernot; Hans Ingelberts

doi:10.1117/12.3022074

19 June 2024 Macroscale fluorescence lifetime imaging with novel time-gated camera complemented with high-resolution color image registration

Sevada Sahakian, Simone Janssen, Thomas Van den Dries, Thomas Lapauw, Marcus Stroet, Théo Lepoutte, Pooria Iranian, Maarten Kuijk, Sophie Hernot, Hans Ingelberts

Author Affiliations +

Proceedings Volume PC13009, Clinical Biophotonics III; PC1300906 (2024) https://doi.org/10.1117/12.3022074
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Macroscale fluorescence lifetime (FLT) imaging is emerging as a promising tool to improve tumor margin delineation and enhance contrast between tumor and healthy tissue during fluorescence-guided surgery. We have been developing the tauCAM, a custom-built time-gated CMOS camera with a high quantum efficiency (QE) in NIR (46%) and a resolution of 128x128 pixels, specifically for this application. The images obtained from the tauCAM are overlaid on high-resolution color images acquired with a dedicated color camera. We demonstrate the capabilities of our camera by performing in vivo studies on subcutaneous mice tumor models administered with nanobody- and antibody-based NIR tumor-targeted fluorescent contrast agents. The results indicate that FLT imaging enhances the contrast between tumor and healthy tissue.

Conference Presentation

Citation Download Citation

Sevada Sahakian, Simone Janssen, Thomas Van den Dries, Thomas Lapauw, Marcus Stroet, Théo Lepoutte, Pooria Iranian, Maarten Kuijk, Sophie Hernot, and Hans Ingelberts "Macroscale fluorescence lifetime imaging with novel time-gated camera complemented with high-resolution color image registration", Proc. SPIE PC13009, Clinical Biophotonics III, PC1300906 (19 June 2024); https://doi.org/10.1117/12.3022074

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