Method for three-dimensional visualization of neurodegeneration in cupric-silver stained serial rat brain slices

Leonid Bunegin; Gleb P. Tolstykh M.D.; Jerry F. Gelineau

doi:10.1117/1.1896006

1 March 2005 Method for three-dimensional visualization of neurodegeneration in cupric-silver stained serial rat brain slices

Leonid Bunegin, Gleb P. Tolstykh M.D., Jerry F. Gelineau

Author Affiliations +

Journal of Biomedical Optics, Vol. 10, Issue 2, 024012 (March 2005). https://doi.org/10.1117/1.1896006

Abstract

The spatial distribution of neurodegeneration in brains is difficult to visualize when working from 2-D serial slices. In studies where repetitive operant behavior measurements are made over several weeks following organic solvent exposure, definitive evidence of degeneration in brain structures may have been significantly cleared by the time the tissue is prepared histologically. The only remaining evidence that injury has occurred may be nothing more than neuronal and cellular debris. By choosing stains that are specific for this type of residual and/or indicative of specific pathology, a 3-D representation of the spatial distribution of the neuronal and cellular debris fields within the organ can be highlighted and displayed. We present a method for visualizing the spatial distribution of neuronal degeneration that can result from low-level organic solvent exposure scenarios. A cupric-silver stain highly specific for neuronal degeneration is used to identify neuronal debris fields in 73 serial slices of brains of rodents that were exposed to toluene vapors. Serial brain sections stained with cupric-silver are scanned at 600 dpi using a gray-scale protocol. Using commercially available software, scans are assembled into 3-D images showing both topographical and internal anatomical details. The reassembled images are further processed into stereo pairs. Gray-scale scans are compared to the original sections to establish gray-scale ranges for healthy and damaged tissue and artifact staining.

©(2005) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Leonid Bunegin, Gleb P. Tolstykh M.D., and Jerry F. Gelineau "Method for three-dimensional visualization of neurodegeneration in cupric-silver stained serial rat brain slices," Journal of Biomedical Optics 10(2), 024012 (1 March 2005). https://doi.org/10.1117/1.1896006

Published: 1 March 2005

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Brain

Tissues

Visualization

3D image processing

Neuroimaging

Neurodegeneration

Statistical analysis

Show All Keywords

RELATED CONTENT

Large scale digital atlases in neuroscience
Proceedings of SPIE (March 21 2014)

Longitudinal intensity normalization of magnetic resonance images using patches
Proceedings of SPIE (March 13 2013)

Three dimensional visualization of neuro degeneration in cupric silver stained...
Proceedings of SPIE (July 09 2003)

Image processing and 3D visualization in forensic pathologic examination
Proceedings of SPIE (February 19 1996)

Techniques for spatial normalization of diffusion tensor images
Proceedings of SPIE (June 06 2000)

Interactive brain shift compensation using GPU based programming
Proceedings of SPIE (February 24 2009)

Reproducibility of MRI segmentation using a feature space method
Proceedings of SPIE (June 24 1998)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years