Imaging of Caenorhabditis elegans neurons by second-harmonic generation and two-photon excitation fluorescence

Giorgos Filippidis; C. Kouloumentas; G. Voglis; F. Zacharopoulou; Theodore G. Papazoglou; N. Tavernarakis

doi:10.1117/1.1886729

1 March 2005 Imaging of Caenorhabditis elegans neurons by second-harmonic generation and two-photon excitation fluorescence

Giorgos Filippidis, C. Kouloumentas, G. Voglis, F. Zacharopoulou, Theodore G. Papazoglou, N. Tavernarakis

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Journal of Biomedical Optics, Vol. 10, Issue 2, 024015 (March 2005). https://doi.org/10.1117/1.1886729

Abstract

Second-harmonic generation (SHG) and two-photon excitation fluorescence (TPEF) are relatively new and promising tools for the detailed imaging of biological samples and processes at the microscopic level. By exploiting these nonlinear phenomena phototoxicity and photobleaching effects on the specimens are reduced dramatically. The main target of this work was the development of a compact inexpensive and reliable experimental apparatus for nonlinear microscopy measurements. Femtosecond laser pulses were utilized for excitation. We achieved high-resolution imaging and mapping of Caenorhabditis elegans (C. elegans) neurons and muscular structures of the pharynx, at the microscopic level by performing SHG and TPEF measurements. By detecting nonlinear phenomena such as SHG and TPEF it is feasible to extract valuable information concerning the structure and the function of nematode neurons.

©(2005) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Giorgos Filippidis, C. Kouloumentas, G. Voglis, F. Zacharopoulou, Theodore G. Papazoglou, and N. Tavernarakis "Imaging of Caenorhabditis elegans neurons by second-harmonic generation and two-photon excitation fluorescence," Journal of Biomedical Optics 10(2), 024015 (1 March 2005). https://doi.org/10.1117/1.1886729

Published: 1 March 2005

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