High-resolution whole organ imaging using two-photon tissue cytometry

Timothy Ragan; Jeremy D. Sylvan; Ki H. Kim; Hayden Huang; Karsten Bahlmann; Richard T. Lee; Peter T. C. So

doi:10.1117/1.2435626

1 January 2007 High-resolution whole organ imaging using two-photon tissue cytometry

Timothy Ragan, Jeremy D. Sylvan, Ki H. Kim, Hayden Huang, Karsten Bahlmann, Richard T. Lee, Peter T. C. So

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Journal of Biomedical Optics, Vol. 12, Issue 1, 014015 (January 2007). https://doi.org/10.1117/1.2435626

Abstract

Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer's disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution. Furthermore, two-photon tissue cytometry offers all the benefits of fluorescence-based approaches including high specificity and sensitivity and appropriateness for molecular imaging of gene and protein expression. We use two-photon tissue cytometry to image an entire mouse heart at subcellular resolution to quantify the 3-D morphology of cardiac microvasculature and myocyte morphology spanning almost five orders of magnitude in length scales.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Timothy Ragan, Jeremy D. Sylvan, Ki H. Kim, Hayden Huang, Karsten Bahlmann, Richard T. Lee, and Peter T. C. So "High-resolution whole organ imaging using two-photon tissue cytometry," Journal of Biomedical Optics 12(1), 014015 (1 January 2007). https://doi.org/10.1117/1.2435626

Published: 1 January 2007

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Cited by 66 scholarly publications and 3 patents.

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KEYWORDS

Tissues

Heart

3D image processing

Image resolution

Tissue optics

Biology

Image segmentation

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