In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation

Wataru Watanabe; Sachhiro Matsunaga; Tsunehito Higashi; Kiichi Fukui; Kazuyoshi Itoh

doi:10.1117/1.2939401

1 May 2008 In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation

Wataru Watanabe, Sachhiro Matsunaga, Tsunehito Higashi, Kiichi Fukui, Kazuyoshi Itoh

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Journal of Biomedical Optics, Vol. 13, Issue 3, 031213 (May 2008). https://doi.org/10.1117/1.2939401

Abstract

Femtosecond laser pulses in the near-infrared region have potential applications in the imaging and manipulation of intracellular organelles. We report on the manipulation of intracellular organelles by two-photon excitation. The dynamics of green fluorescent protein (GFP)-histone are investigated by two-photon fluorescence recovery after photobleaching (FRAP). Intracellular ablation of fluorescently labeled organelles in living cells is performed by focusing femtosecond laser pulses. We report on the selective marking of individual organelles by using two-photon conversion of a photoconvertible fluorescent protein.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Wataru Watanabe, Sachhiro Matsunaga, Tsunehito Higashi, Kiichi Fukui, and Kazuyoshi Itoh "In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation," Journal of Biomedical Optics 13(3), 031213 (1 May 2008). https://doi.org/10.1117/1.2939401

Published: 1 May 2008

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