Long-working-distance fluorescence microscope with high-numerical-aperture objectives for variable-magnification imaging in live mice from macro- to subcellular

Hiroaki Kimura; Masashi Momiyama; Katsuro Tomita; Hiroyuki Tsuchiya; Robert M. Hoffman

doi:10.1117/1.3526356

1 November 2010 Long-working-distance fluorescence microscope with high-numerical-aperture objectives for variable-magnification imaging in live mice from macro- to subcellular

Hiroaki Kimura, Masashi Momiyama, Katsuro Tomita, Hiroyuki Tsuchiya, Robert M. Hoffman

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Journal of Biomedical Optics, Vol. 15, Issue 6, 066029 (November 2010). https://doi.org/10.1117/1.3526356

Abstract

We demonstrate the development of a long-working-distance fluorescence microscope with high-numerical-aperture objectives for variable-magnification imaging in live mice from macro- to subcellular. To observe cytoplasmic and nuclear dynamics of cancer cells in the living mouse, 143B human osteosarcoma cells are labeled with green fluorescent protein in the nucleus and red fluorescent protein in the cytoplasm. These dual-color cells are injected by a vascular route in an abdominal skin flap in nude mice. The mice are then imaged with the Olympus MVX10 macroview fluorescence microscope. With the MVX10, the nuclear and cytoplasmic behavior of cancer cells trafficking in blood vessels of live mice is observed. We also image lung metastases in live mice from the macro- to the subcellular level by opening the chest wall and imaging the exposed lung in live mice. Injected splenocytes, expressing cyan fluorescent protein, could also be imaged on the lung of live mice. We demonstrate that the MVX10 microscope offers the possibility of full-range in vivo fluorescence imaging from macro- to subcellular and should enable widespread use of powerful imaging technologies enabled by genetic reporters and other fluorophores.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Hiroaki Kimura, Masashi Momiyama, Katsuro Tomita, Hiroyuki Tsuchiya, and Robert M. Hoffman "Long-working-distance fluorescence microscope with high-numerical-aperture objectives for variable-magnification imaging in live mice from macro- to subcellular," Journal of Biomedical Optics 15(6), 066029 (1 November 2010). https://doi.org/10.1117/1.3526356

Published: 1 November 2010

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