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1 November 2011 Single myelin fiber imaging in living rodents without labeling by deep optical coherence microscopy
Juliette Ben Arous, Jonas Binding, Jean-Francois Leger, Mariano Casado, Piotr Topilko, Laurent Bourdieu, Sylvain Gigan, A. Claude Boccara
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Journal of Biomedical Optics, Vol. 16, Issue 11, 116012 (November 2011). https://doi.org/10.1117/1.3650770
Abstract
Myelin sheath disruption is responsible for multiple neuropathies in the central and peripheral nervous system. Myelin imaging has thus become an important diagnosis tool. However, in vivo imaging has been limited to either low-resolution techniques unable to resolve individual fibers or to low-penetration imaging of single fibers, which cannot provide quantitative information about large volumes of tissue, as required for diagnostic purposes. Here, we perform myelin imaging without labeling and at micron-scale resolution with <300-μm penetration depth on living rodents. This was achieved with a prototype [termed deep optical coherence microscopy (deep-OCM)] of a high-numerical aperture infrared full-field optical coherence microscope, which includes aberration correction for the compensation of refractive index mismatch and high-frame-rate interferometric measurements. We were able to measure the density of individual myelinated fibers in the rat cortex over a large volume of gray matter. In the peripheral nervous system, deep-OCM allows, after minor surgery, in situ imaging of single myelinated fibers over a large fraction of the sciatic nerve. This allows quantitative comparison of normal and Krox20 mutant mice, in which myelination in the peripheral nervous system is impaired. This opens promising perspectives for myelin chronic imaging in demyelinating diseases and for minimally invasive medical diagnosis.
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2011/16(11)/116012/9/$25.00
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Juliette Ben Arous, Jonas Binding, Jean-Francois Leger, Mariano Casado, Piotr Topilko, Laurent Bourdieu, Sylvain Gigan, and A. Claude Boccara "Single myelin fiber imaging in living rodents without labeling by deep optical coherence microscopy," Journal of Biomedical Optics 16(11), 116012 (1 November 2011). https://doi.org/10.1117/1.3650770
Published: 1 November 2011
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KEYWORDS
In vivo imaging
Infrared imaging
Nerve
Brain
Cameras
In vitro testing
Image resolution
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