Understanding three-dimensional spatial relationship between the mouse second polar body and first cleavage plane with full-field optical coherence tomography

Jing-gao Zheng; Tiancheng Huo; Tianyuan Chen; Chengming Wang; Ning Zhang; Ning Tian; Fengying Zhao; Dieyan Chen; Wanyun Ma; Jia-Lin Sun; Ping Xue; Danyu Lu

doi:10.1117/1.JBO.18.1.010503

13 December 2012 Understanding three-dimensional spatial relationship between the mouse second polar body and first cleavage plane with full-field optical coherence tomography

Jing-gao Zheng, Tiancheng Huo, Tianyuan Chen, Chengming Wang, Ning Zhang, Ning Tian, Fengying Zhao, Dieyan Chen, Wanyun Ma, Jia-Lin Sun, Ping Xue, Danyu Lu

Author Affiliations +

Journal of Biomedical Optics, Vol. 18, Issue 1, 010503 (December 2012). https://doi.org/10.1117/1.JBO.18.1.010503

Abstract

The morphogenetic relationship between early patterning and polarity formation is of fundamental interest and remains a controversial issue in preimplantation embryonic development. We use a label-free three-dimensional (3-D) imaging technique of full-field optical coherence tomography (FF-OCT) successfully for the first time to study the dynamics of developmental processes in mouse preimplantation lives. Label-free 3-D subcellular time-lapse images are demonstrated to investigate 3-D spatial relationship between the second polar body (2PB) and the first cleavage plane. By using FF-OCT together with quantitative study, we show that only 25% of the predicted first cleavage planes, defined by the apposing plane of two pronuclei, pass through the 2PB. Also only 27% of the real cleavage planes pass through the 2PB. These results suggest that the 2PB is not a convincing spatial cue for the event of the first cleavage. Our studies demonstrate the feasibility of FF-OCT in providing new insights and potential breakthroughs to the controversial issues of early patterning and polarity in mammalian developmental biology.

Citation Download Citation

Jing-gao Zheng, Tiancheng Huo, Tianyuan Chen, Chengming Wang, Ning Zhang, Ning Tian, Fengying Zhao, Dieyan Chen, Wanyun Ma, Jia-Lin Sun, Ping Xue, and Danyu Lu "Understanding three-dimensional spatial relationship between the mouse second polar body and first cleavage plane with full-field optical coherence tomography," Journal of Biomedical Optics 18(1), 010503 (13 December 2012). https://doi.org/10.1117/1.JBO.18.1.010503

Published: 13 December 2012

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY