Semiautomatic and rapid quantification of heartbeat parameters in Drosophila using optical coherence tomography imaging

Shou-Yuan Guo; Fang-Tsu Liao; Ming-Tsan Su; Cheng-Yi Chang; Hong-Ren Su; Jyun-Cin Huang; Wen-Chuan Kuo

doi:10.1117/1.JBO.18.2.026004

1 February 2013 Semiautomatic and rapid quantification of heartbeat parameters in Drosophila using optical coherence tomography imaging

Shou-Yuan Guo, Fang-Tsu Liao, Ming-Tsan Su, Cheng-Yi Chang, Hong-Ren Su, Jyun-Cin Huang, Wen-Chuan Kuo

Author Affiliations +

Funded by: National Science Council, National Health Research Institute

Journal of Biomedical Optics, Vol. 18, Issue 2, 026004 (February 2013). https://doi.org/10.1117/1.JBO.18.2.026004

Abstract

We report a semiautomatic algorithm that is specialized for rapid analysis of beat-to-beat contraction-relaxation parameters of the heart in Drosophila. The presented algorithm adapts the general graph theoretical image segmentation algorithm and a histogram-based thresholding algorithm, which can measure many cardiac parameters, including heart rate, heart period, diastolic and systolic intervals, and end-diastolic and end-systolic areas. Additionally, dynamic cardiac functions, such as arrhythmia index and percent fractional shortening, can be automatically calculated for all the recorded heartbeats over significant periods of time.

© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Shou-Yuan Guo, Fang-Tsu Liao, Ming-Tsan Su, Cheng-Yi Chang, Hong-Ren Su, Jyun-Cin Huang, and Wen-Chuan Kuo "Semiautomatic and rapid quantification of heartbeat parameters in Drosophila using optical coherence tomography imaging," Journal of Biomedical Optics 18(2), 026004 (1 February 2013). https://doi.org/10.1117/1.JBO.18.2.026004

Published: 1 February 2013

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KEYWORDS

Image segmentation

Heart

Optical coherence tomography

Evolutionary algorithms

Image processing algorithms and systems

Coherence imaging

Algorithms

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