Direct trabecular meshwork imaging in porcine eyes through multiphoton gonioscopy

Omid Masihzadeh; David A. Ammar; Malik Y. Kahook M.D.; Emily A. Gibson; Tim C. Lei

doi:10.1117/1.JBO.18.3.036009

20 March 2013 Direct trabecular meshwork imaging in porcine eyes through multiphoton gonioscopy

Omid Masihzadeh, David A. Ammar, Malik Y. Kahook M.D., Emily A. Gibson, Tim C. Lei

Author Affiliations +

Funded by: University of Colorado Technology Transfer Office

Journal of Biomedical Optics, Vol. 18, Issue 3, 036009 (March 2013). https://doi.org/10.1117/1.JBO.18.3.036009

Abstract

The development of technologies to characterize the ocular aqueous outflow system (AOS) is important for the understanding of the pathophysiology of glaucoma. Multiphoton microscopy (MPM) offers the advantage of high-resolution, label-free imaging with intrinsic image contrast because the emitted signals result from the specific biomolecular content of the tissue. Previous attempts to use MPM to image the murine irido-corneal region directly through the sclera have suffered from degradation in image resolution due to scattering of the focused laser light. As a result, transscleral MPM has limited ability to observe fine structures in the AOS. In this work, the porcine irido-corneal angle was successfully imaged through the transparent cornea using a gonioscopic lens to circumvent the highly scattering scleral tissue. The resulting high-resolution images allowed the detailed structures in the trabecular meshwork (TM) to be observed. Multimodal imaging by two-photon autofluorescence and second harmonic generation allowed visualization of different features in the TM without labels and without disruption of the TM or surrounding tissues. MPM gonioscopy is a promising noninvasive imaging tool for high-resolution studies of the AOS, and research continues to explore the potential for future clinical applications in humans.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Omid Masihzadeh, David A. Ammar, Malik Y. Kahook M.D., Emily A. Gibson, and Tim C. Lei "Direct trabecular meshwork imaging in porcine eyes through multiphoton gonioscopy," Journal of Biomedical Optics 18(3), 036009 (20 March 2013). https://doi.org/10.1117/1.JBO.18.3.036009

Published: 20 March 2013

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