Four-dimensional motility tracking of biological cells by digital holographic microscopy

Xiao Yu; Jisoo Hong; Changgeng Liu; Michael Cross; Donald T. Haynie; Myung K. Kim

doi:10.1117/1.JBO.19.4.045001

3 April 2014 Four-dimensional motility tracking of biological cells by digital holographic microscopy

Xiao Yu, Jisoo Hong, Changgeng Liu, Michael Cross, Donald T. Haynie, Myung K. Kim

Author Affiliations +

Journal of Biomedical Optics, Vol. 19, Issue 4, 045001 (April 2014). https://doi.org/10.1117/1.JBO.19.4.045001

Abstract

Three-dimensional profiling and tracking by digital holography microscopy (DHM) provide label-free and quantitative analysis of the characteristics and dynamic processes of objects, since DHM can record real-time data for microscale objects and produce a single hologram containing all the information about their three-dimensional structures. Here, we have utilized DHM to visualize suspended microspheres and microfibers in three dimensions, and record the four-dimensional trajectories of free-swimming cells in the absence of mechanical focus adjustment. The displacement of microfibers due to interactions with cells in three spatial dimensions has been measured as a function of time at subsecond and micrometer levels in a direct and straightforward manner. It has thus been shown that DHM is a highly efficient and versatile means for quantitative tracking and analysis of cell motility.

Citation Download Citation

Xiao Yu, Jisoo Hong, Changgeng Liu, Michael Cross, Donald T. Haynie, and Myung K. Kim "Four-dimensional motility tracking of biological cells by digital holographic microscopy," Journal of Biomedical Optics 19(4), 045001 (3 April 2014). https://doi.org/10.1117/1.JBO.19.4.045001

Published: 3 April 2014

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY