Limited-projection-angle hybrid fluorescence molecular tomography of multiple molecules

Karin Radrich; Pouyan Mohajerani; Johanna Bussemer; Markus Schwaiger; Ambros J. Beer; Vasilis Ntziachristos

doi:10.1117/1.JBO.19.4.046016

25 April 2014 Limited-projection-angle hybrid fluorescence molecular tomography of multiple molecules

Karin Radrich, Pouyan Mohajerani, Johanna Bussemer, Markus Schwaiger, Ambros J. Beer, Vasilis Ntziachristos

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Journal of Biomedical Optics, Vol. 19, Issue 4, 046016 (April 2014). https://doi.org/10.1117/1.JBO.19.4.046016

Abstract

An advantage of fluorescence methods over other imaging modalities is the ability to concurrently resolve multiple moieties using fluorochromes emitting at different spectral regions. Simultaneous imaging of spectrally separated agents is helpful in interrogating multiple functions or establishing internal controls for accurate measurements. Herein, we investigated multimoiety imaging in the context of a limited-projection-angle hybrid fluorescence molecular tomography (FMT), and x-ray computed tomography implementation and the further registration with positron emission tomography (PET) data. Multichannel FMT systems may image fluorescent probes of varying distribution patterns. Therefore, it is possible that different channels may require different use of priors and regularization parameters. We examined the performance of automatically estimating regularization factors implementing priors, using data-driven regularization specific for limited-projection-angle schemes. We were particularly interested in identifying the implementation variations between hybrid-FMT channels due to probe distribution variation. For this reason, initial validation of the data-driven algorithm on a phantom was followed by imaging different agent distributions in animals, assuming superficial and deep seated activity. We further demonstrate the benefits of combining hybrid FMT with PET to gain multiple readings on the molecular composition of disease.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Karin Radrich, Pouyan Mohajerani, Johanna Bussemer, Markus Schwaiger, Ambros J. Beer, and Vasilis Ntziachristos "Limited-projection-angle hybrid fluorescence molecular tomography of multiple molecules," Journal of Biomedical Optics 19(4), 046016 (25 April 2014). https://doi.org/10.1117/1.JBO.19.4.046016

Published: 25 April 2014

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