Phantom validation of Monte Carlo modeling for noncontact depth sensitive fluorescence measurements in an epithelial tissue model

Yi Hong Ong; Caigang Zhu; Quan Liu

doi:10.1117/1.JBO.19.8.085006

12 August 2014 Phantom validation of Monte Carlo modeling for noncontact depth sensitive fluorescence measurements in an epithelial tissue model

Yi Hong Ong, Caigang Zhu, Quan Liu

Author Affiliations +

Funded by: Ministry of Education, Ministry of Education in Singapore, National Research Foundation, National Research Foundation (NRF) and ASTAR-PSF, ASTAR-SERC in Singapore, ASTAR-SERC

Journal of Biomedical Optics, Vol. 19, Issue 8, 085006 (August 2014). https://doi.org/10.1117/1.JBO.19.8.085006

Abstract

Experimental investigation and optimization of various optical parameters in the design of depth sensitive optical measurements in layered tissues would require a huge amount of time and resources. A computational method to model light transport in layered tissues using Monte Carlo simulations has been developed for decades to reduce the cost incurred during this process. In this work, we employed the Monte Carlo method to investigate the depth sensitivity achieved by various illumination and detection configurations including both the traditional cone configurations and new cone shell configurations, which are implemented by convex or axicon lenses. Phantom experiments have been carried out to validate the Monte Carlo modeling of fluorescence in a two-layered turbid, epithelial tissue model. The measured fluorescence and depth sensitivity of different illumination–detection configurations were compared with each other. The results indicate excellent agreement between the experimental and simulation results in the trends of fluorescence intensity and depth sensitivity. The findings of this study and the development of the Monte Carlo method for noncontact setups provide useful insight and assistance in the planning and optimization of optical designs for depth sensitive fluorescence measurements.

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Yi Hong Ong, Caigang Zhu, and Quan Liu "Phantom validation of Monte Carlo modeling for noncontact depth sensitive fluorescence measurements in an epithelial tissue model," Journal of Biomedical Optics 19(8), 085006 (12 August 2014). https://doi.org/10.1117/1.JBO.19.8.085006

Published: 12 August 2014

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KEYWORDS

Monte Carlo methods

Axicons

Luminescence

Tissues

Tissue optics

Lenses

Absorption

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