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25 February 2015 Enhanced resolution through thick tissue with structured illumination and adaptive optics
Benjamin Thomas, Adrian Wolstenholme, Snehal N. Chaudhari, Edward T. Kipreos, Peter Kner
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Journal of Biomedical Optics, Vol. 20, Issue 2, 026006 (February 2015). https://doi.org/10.1117/1.JBO.20.2.026006
Abstract
Structured illumination microscopy provides twice the linear resolution of conventional fluorescence microscopy, but in thick samples, aberrations degrade the performance and limit the resolution. Here, we demonstrate structured illumination microscopy through 35 µm of tissue using adaptive optics (AO) to correct aberrations resulting in images with a resolution of 140 nm. We report a 60% minimum improvement in the signal-to-noise ratio of the structured illumination reconstruction through thick tissue by correction with AO.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2015/$25.00 © 2015 SPIE
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Benjamin Thomas, Adrian Wolstenholme, Snehal N. Chaudhari, Edward T. Kipreos, and Peter Kner "Enhanced resolution through thick tissue with structured illumination and adaptive optics," Journal of Biomedical Optics 20(2), 026006 (25 February 2015). https://doi.org/10.1117/1.JBO.20.2.026006
Published: 25 February 2015
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KEYWORDS
Adaptive optics
Microscopy
Wavefronts
Tissue optics
Signal to noise ratio
Image resolution
Resolution enhancement technologies
CHORUS Article. This article was made freely available starting 25 February 2016
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