High-refractive index of acrylate embedding resin clarifies mouse brain tissue

Hongfu Zhou; Yumiao Xiong; Yu Wang; Xiaojun Wang; Pei Li; Yadong Gang; Xiuli Liu; Shaoqun Zeng

doi:10.1117/1.JBO.22.11.110503

16 November 2017 High-refractive index of acrylate embedding resin clarifies mouse brain tissue

Hongfu Zhou, Yumiao Xiong, Yu Wang, Xiaojun Wang, Pei Li, Yadong Gang, Xiuli Liu, Shaoqun Zeng

Author Affiliations +

Journal of Biomedical Optics, Vol. 22, Issue 11, 110503 (November 2017). https://doi.org/10.1117/1.JBO.22.11.110503

Abstract

Biological tissue transparency combined with light-sheet fluorescence microscopy is a useful method for studying the neural structure of biological tissues. The development of light-sheet fluorescence microscopy also promotes progress in biological tissue clearing methods. The current clarifying methods mostly use liquid reagent to denature protein or remove lipids first, to eliminate or reduce the scattering index or refractive index of the biological tissue. However, denaturing protein and removing lipids require complex procedures or an extended time period. Therefore, here we have developed acrylate resin with a high refractive index, which causes clearing of biological tissue directly after polymerization. This method can improve endogenous fluorescence retention by adjusting the pH value of the resin monomer.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Hongfu Zhou, Yumiao Xiong, Yu Wang, Xiaojun Wang, Pei Li, Yadong Gang, Xiuli Liu, and Shaoqun Zeng "High-refractive index of acrylate embedding resin clarifies mouse brain tissue," Journal of Biomedical Optics 22(11), 110503 (16 November 2017). https://doi.org/10.1117/1.JBO.22.11.110503

Received: 5 July 2017; Accepted: 6 October 2017; Published: 16 November 2017

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY