Line-field confocal optical coherence tomography for high-resolution noninvasive imaging of skin tumors

Arnaud Dubois; Olivier Levecq; Hicham Azimani; David Siret; Anaïs Barut; Mariano Suppa; Véronique del Marmol; Josep Malvehy; Elisa Cinotti; Pietro Rubegni; Jean-Luc Perrot

doi:10.1117/1.JBO.23.10.106007

23 October 2018 Line-field confocal optical coherence tomography for high-resolution noninvasive imaging of skin tumors

Arnaud Dubois, Olivier Levecq, Hicham Azimani, David Siret, Anaïs Barut, Mariano Suppa, Véronique del Marmol, Josep Malvehy, Elisa Cinotti, Pietro Rubegni, Jean-Luc Perrot

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Journal of Biomedical Optics, Vol. 23, Issue 10, 106007 (October 2018). https://doi.org/10.1117/1.JBO.23.10.106007

Abstract

An optical technique called line-field confocal optical coherence tomography (LC-OCT) is introduced for high-resolution, noninvasive imaging of human skin in vivo. LC-OCT combines the principles of time-domain optical coherence tomography and confocal microscopy with line illumination and detection using a broadband laser and a line-scan camera. LC-OCT measures the echo-time delay and amplitude of light backscattered from cutaneous microstructures through low-coherence interferometry associated with confocal spatial filtering. Multiple A-scans are acquired simultaneously while dynamically adjusting the focus. The resulting cross-sectional B-scan image is produced in real time at 10 frame / s. With an isotropic spatial resolution of ∼1 μm, the LC-OCT images reveal a comprehensive structural mapping of skin at the cellular level down to a depth of ∼500 μm. LC-OCT has been applied to the imaging of various skin lesions, in vivo, including carcinomas and melanomas. LC-OCT images are found to strongly correlate with conventional histopathological images. The use of LC-OCT as an adjunct tool in medical practice could significantly improve clinical diagnostic accuracy while reducing the number of biopsies of benign lesions.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Arnaud Dubois, Olivier Levecq, Hicham Azimani, David Siret, Anaïs Barut, Mariano Suppa, Véronique del Marmol, Josep Malvehy, Elisa Cinotti, Pietro Rubegni, and Jean-Luc Perrot "Line-field confocal optical coherence tomography for high-resolution noninvasive imaging of skin tumors," Journal of Biomedical Optics 23(10), 106007 (23 October 2018). https://doi.org/10.1117/1.JBO.23.10.106007

Received: 17 July 2018; Accepted: 1 October 2018; Published: 23 October 2018

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