In vivo multiphoton kinetic imaging of the toxic effect of carbon tetrachloride on hepatobiliary metabolism

Chih-Ju Lin; Sheng-Lin Lee; Hsuan-Shu Lee; Chen-Yuan Dong

doi:10.1117/1.JBO.23.9.091407

25 June 2018 In vivo multiphoton kinetic imaging of the toxic effect of carbon tetrachloride on hepatobiliary metabolism

Chih-Ju Lin, Sheng-Lin Lee, Hsuan-Shu Lee, Chen-Yuan Dong

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Journal of Biomedical Optics, Vol. 23, Issue 9, 091407 (June 2018). https://doi.org/10.1117/1.JBO.23.9.091407

Abstract

We used intravital multiphoton microscopy to study the recovery of hepatobiliary metabolism following carbon tetrachloride (CCl₄) induced hepatotoxicity in mice. The acquired images were processed by a first order kinetic model to generate rate constant resolved images of the mouse liver. We found that with progression of hepatotoxicity, the spatial gradient of hepatic function disappeared. A CCl₄-induced damage mechanism involves the compromise of membrane functions, resulting in accumulation of processed 6-carboxyfluorescein molecules. At day 14 following induction, a restoration of the mouse hepatobiliary function was found. Our approach allows the study of the response of hepatic functions to chemical agents in real time and is useful for studying pharmacokinetics of drug molecules through optical microscopic imaging.

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Chih-Ju Lin, Sheng-Lin Lee, Hsuan-Shu Lee, and Chen-Yuan Dong "In vivo multiphoton kinetic imaging of the toxic effect of carbon tetrachloride on hepatobiliary metabolism," Journal of Biomedical Optics 23(9), 091407 (25 June 2018). https://doi.org/10.1117/1.JBO.23.9.091407

Received: 13 November 2017; Accepted: 23 April 2018; Published: 25 June 2018

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