Quantitative estimation of optical properties in bilayer media within the subdiffusive regime using tilted fiber-optic probe diffuse reflectance spectroscopy, part 2: probe design, realization, and experimental validation

Philippe De Tillieux; Maxime Baillot; Pierre Marquet

doi:10.1117/1.JBO.29.10.105002

29 October 2024 Quantitative estimation of optical properties in bilayer media within the subdiffusive regime using tilted fiber-optic probe diffuse reflectance spectroscopy, part 2: probe design, realization, and experimental validation

Philippe De Tillieux, Maxime Baillot, Pierre Marquet

Author Affiliations +

Journal of Biomedical Optics, Vol. 29, Issue 10, 105002 (October 2024). https://doi.org/10.1117/1.JBO.29.10.105002

Abstract

Significance

Tissues like skin have a layered structure where each layer's optical properties vary significantly. However, traditional diffuse reflectance spectroscopy assumes a homogeneous medium, often leading to estimations that reflect the properties of neither layer. There's a clear need for probes that can precisely measure the optical properties of layered tissues.

Aim

This paper aims to design a diffuse reflectance probe capable of accurately estimating the optical properties of bilayer tissues in the subdiffusive regime.

Approach

Using Monte Carlo simulations, we evaluated key geometric factors—fiber placement, tilt angle, diameter, and numerical aperture—on optical property estimation, following the methodology in Part I. A robust design is proposed that balances accurate intrinsic optical property (IOP) calculations with practical experimental constraints.

Results

The designed probe, featuring eight illumination and eight detection fibers with varying spacings and tilt angles. The estimation error of the IOP calculation for bilayer phantoms is less than 20% for top layers with thicknesses between 0.2 and 1.0 mm.ConclusionBuilding on the approach from Part I and using a precise calibration, the probe effectively quantified and distinguished the IOPs of bilayer samples, particularly those relevant to early skin pathology detection and characterization.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Philippe De Tillieux, Maxime Baillot, and Pierre Marquet "Quantitative estimation of optical properties in bilayer media within the subdiffusive regime using tilted fiber-optic probe diffuse reflectance spectroscopy, part 2: probe design, realization, and experimental validation," Journal of Biomedical Optics 29(10), 105002 (29 October 2024). https://doi.org/10.1117/1.JBO.29.10.105002

Received: 15 May 2024; Accepted: 16 September 2024; Published: 29 October 2024

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KEYWORDS

Error analysis

Optical properties

Photons

Simulations

Melanoma

Tissues

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