Described in this study is an advanced co-axial acoustic stimulation technique with pre-compensation for acoustic frequency content and reconstruction of signals in the Fourier domain for use in Optical Coherence Tomography (OCT) Vibrography. The feasibility of the technique was demonstrated via the measurement of the first mechanical, flexural resonance modes of two contact lenses with varied elastic moduli and an ex-vivo porcine cornea, each with a maintained constant intraocular pressure. The measurement of these resonance modes was achieved through use of a Swept Source OCT system, operated in phase sensitive mode, to detect the nanometer scale displacements of these modes.
Corneal cross-linking (CXL) has grown from an interesting concept to a practical clinical treatment for corneal ectatic disease globally in the past three decades. In both understanding the principle of how CXL proceeds and monitoring the clinical procedure, detection of structural changes during cornea CXL plays a significant role. This paper demonstrates a novel over-sampling nano-sensitive optical coherence tomography (osnsOCT) method, which is potential to detect nanoscale structural changes in various tissues, to simultaneously measure the structural variations during the corneal CXL treatment.
In biomedical optics, Optical Coherence Tomography (OCT) is an emerging optical imaging modality during the last three decades. OCT can perform high resolution, cross-section imaging of the internal microstructure in biological tissues by measuring echoes of backscattered light. We have developed a broadband, high-resolution spectral domain OCT system whose central wavelength is 1300 nm with bandwidth ~400 nm. Theoretical axial resolution of the system is ~3.71 μm and experimentally we get ~5 μm in air. Furthermore, we will apply this system for nano-sensitive detection and visualization in varieties of biological tissues.
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