The nucleotide sequences of the GPCR gene of Lumpy Skin Disease Virus (LSDV) and Sheeppox Virus (SPPV) strains have been transformed into 2D realizations of GB-speckles (Gene-Based speckles). Based on the analysis of statistical properties of corresponding GB-speckles, a possibility of differentiation between wild field LSDV and the SPPV strains which could be used as heterologous vaccines against Lumpy Skin Disease has been shown. It has been demonstrated that an application of the GB-speckles has the great perspectives from viewpoint of modern bioinformatics.
As it has been demonstrated earlier, different types of re-coding of nucleotide sequences to gene-based speckle-patterns (GB-speckles) can be used. In this paper an advanced study, devoted to optimization of this algorithm, is presented. With this purpose, investigations of statistical properties of the GB-speckles, generated on the nucleotide sequences of the fumC gene of Chlamydia trachomatis, have been performed. The first- and the second-order statistics of intensity fluctuations in GB-speckles have been analyzed. Perspectives of the gene identification using a statistical analysis of laser GB-speckles have been demonstrated. The analyzed nucleotide sequences of the fumC gene of C. trachomatis strains have been isolated in the Republic of Belarus.
Gene-based speckles (GB-speckles) have been implied for interpretation of nucleotide sequences of the target genes of Avian Influenza Virus (AIV). The 1st and 2nd order statistics of 2D speckle structures corresponding to the nucleotide sequences of AIV have been analyzed. It has been shown that these GB-speckles are forming in the case of small number of scatterers and they obey to non-Gaussian statistics. These GB-speckles are characterizing by essential spatial inhomogeneity. Potentials of proposed laser speckle technique for the AIV target genes identification are demonstrated.
Methods of t-LASCA and s-LASCA imaging have been adapted to the problem of monitoring of blood microcirculation in naïve chicken embryo. Set-up for LASCA imaging of the model chicken embryo is mounted. The novel original technique of preparing of optical windows for observation of a naïve chicken embryo is suggested. This technique is based on a removal of part of the natural egg shell using a citric acid solution with further optical clearing using glycerol and glucose.
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