Background. The aim of our study was to estimate the association of lipid fractions and skin autofluorescence (AF), measured with experimental sample of fiber optic spectrometer FOS-1, in patients with diabetes mellitus (DM). Methods. We included in the study 44 type 1 DM patients (median age 29.5 (24.0; 36.0) and 23 type 2 DM patients, median age 58.0 (47.0; 61.0). Blood lipids and glycosylated hemoglobin (HbA1c) were analyzed. Skin AF was measured with fluorescence–reflection spectrometer FOS-1 at a wavelength of 460 nm with excitation of 365 nm. Ratio of fluorescent signal to signal of reflection in excitation region was used as measured parameter to reduce the effect of skin pigmentation. The measurements were carried out at 5 points on the skin area of forearms. Results. Type 1 DM group was divided to 13 dyslipidemic and 31 normolipidemic patients, subgroups were comparable by age, DM duration and HbA1c level. AF level in dyslipidemic and normolipidemic patients was 0.95 (0.82; 1.16) arb. units and 0.86 (0.79; 0.91) arb. units respectively, p=0.016. In type 1 DM group we found significant positive correlation between skin AF and total cholesterol level (R=0.45, р<0.05) and triglycerides level (R=0.57, p<0.05). In type 2 DM group no lipid fraction showed significant positive correlation with skin AF level. Conclusion. The spectrometer FOS-1 can be used for additional risk evaluation in young and middle-aged type 1 DM patients, but simultaneous presence of several risk factors in older group of type 2 DM patients obstruct the use of the method.
Introduction. Fluorescence spectrometry allows studying skin autofluorescence (AF), which shows content of advanced glycation end products. The aim of study was to determine possibility of using the experimental sample of fiber optic spectrometer FOS-1 for diabetes mellitus diagnostics and control, including noninvasive diagnostics of complications.
Methods. We involved 36 healthy participants, 13 type 1 diabetic patients, 10 type 2 diabetic patients. The 1st and the 2nd groups were comparable by the age, gender, skin reflection coefficient, characterizing skin phototype and degree of tanning. Skin AF was measured at a wavelength of 460 nm with excitation of 365 nm. To reduce effect of skin pigmentation, ratio of fluorescent signal to signal of reflection in excitation region was used as measured parameter.
Results. Significant correlation between AF intensity and age was found in type 1 diabetic and control groups (R=0.6, р<0.05 and R=0.43, p<0.05, respectively). No significant difference in AF level was found between these groups: median AF was 0.87 (0.86; 0.89) arb. units and 0.85 (0.77; 0.88) arb. units respectively. In type 1 diabetic group AF also positively correlated, although not statistically significantly, with diabetes duration, glycosylated hemoglobin level, average daily glucose level (R=0.52, p=0.06; R=0.45, p=0.09 and R=0.56, p=0.07 respectively). The median AF was 14.7% higher (p=0.34) in patients with several diabetes complications than in diabetics with 1 complication and 13.9% (р=0.19) higher than in patients without complications.
Conclusion. Obtained data show possibility of using the described method with spectrometer FOS-1 for diabetes control and for diagnostics of microvascular complications.
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