Ms. Hua Mi Profile

Hua Mi

at City Univ of Hong Kong

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Proceedings Article | 3 October 2023 Poster + Paper

Plasmonic metallic nanoparticle-based biosensors

Jung-hoon Lee, Hua Mi

Proceedings Volume 12654, 126540B (2023) https://doi.org/10.1117/12.2676310

KEYWORDS: Magnetism, Gold nanoparticles, Gold, Plasmonics, Polymerase chain reaction, Iron, Fluorescence, Target detection, Signal detection, Nanomaterials

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Bifunctional nanoparticle of combining magnetic and plasmonic nanomaterials retain both unique properties, contributing to the high photothermal performance, excellent biocompatibility, physiological stability, low cytotoxicity and easy separation. Herein, we report a core-shell plasmonic magnetic nanostructure (PMNs), then introduce the plasmonic photothermal polymerase chain reaction (PPT-PCR) platform for fast, sensitive, cheap, and simple nucleic acid detection based on PMNs. Magnetic nanoparticles can be synthesized by solvothermal reaction. PMNs can be prepared after Au coating on the magnetic core, which can act as nanoheater and heat solution to 95°C in several seconds upon infra-red (IR) light irradiation, and can be collected by magnet easily. Furthermore, our platforms utilize ultrafast PCR amplification based on the photothermal effect of plasmonic magnetic nanoparticles for molecular diagnostics through two modes, including in-situ end-point quantitative fluorescence detection (PPT-qPCR) and colorimetric assay (PPT-cPCR), having comparable limit of detection (LOD) on DNA targets.

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