In support of previous cell culture work, we wanted to determine if cell density has an effect on the temperature at which cells die. We used the method of microthermography because it provided threshold temperatures. By not using laser dose, we avoided complications from varying absorptivity and diffusivity of samples. Microthermography looks at temperature at the boundary between live and dead cells using a high-speed mid-IR camera and high-magnification optics. The temperature at the boundary of cell death is independent of the irradiance and represents the true biological threshold regardless of the size of damaged area. Using a 2-um laser, we explored whether the boundary of cell death, and thus the damage rate process, is dependent on cell confluency.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.