Dr. Jozsef Bocsi Profile

Dr. Jozsef Bocsi

at Univ Leipzig

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Publications (20)

Proceedings Article | 5 March 2015 Paper

Standardizing flow cytometric assays in long-term population-based studies

Susanne Melzer, Jozsef Bocsi, Attila Tárnok

Proceedings Volume 9315, 93150H (2015) https://doi.org/10.1117/12.2075426

KEYWORDS: Blood, Analytical research, Data analysis, Statistical analysis, Error analysis, Flow cytometry, Luminescence, Laser stabilization, Biological research, Calibration

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Proceedings Article | 4 March 2014 Paper

Diagnosis of myocardial infarction based on lectin-induced erythrocyte agglutination: a feasibility study

József Bocsi, Kathleen Nieschke, Anja Mittag, Thomas Reichert, Wiebke Laffers, Monika Marecka, Arkadiusz Pierzchalski, Joachim Piltz, Hans-Jürgen Esche, Günther Wolf, Ingo Dähnert, Adolf Baumgartner, Attila Tarnok

Proceedings Volume 8947, 89470V (2014) https://doi.org/10.1117/12.2037702

KEYWORDS: Blood, Absorbance, Diagnostics, Particles, Chest, Light scattering, Heart, Mathematical modeling, Electrocardiography, Proteins

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Myocardial infarction (MI) is an acute life-threatening disease with a high incidence worldwide. Aim of this study was to test lectin-carbohydrate binding-induced red blood cell (RBC) agglutination as an innovative tool for fast, precise and cost effective diagnosis of MI. Five lectins (Ricinus communis agglutinin (RCA), Phaseolus vulgaris erythroagglutinin (PHA), Datura stramonium agglutinin (DSA), Artocarpus agglutinin (ArA), Triticum agglutinin (TA)) were tested for ability to differentiate between agglutination characteristics in patients with MI (n = 101) or angina pectoris without MI (AP) (n = 34) and healthy volunteers (HV) as control (n =68) . RBC agglutination was analyzed by light absorbance of a stirred RBC suspension in the green to red light spectrum in an agglutimeter (amtec, Leipzig, Germany) for 15 min after lectin addition. Mean cell count in aggregates was estimated from light absorbance by a mathematical model. Each lectin induced RBC agglutination. RCA led to the strongest RBC agglutination (~500 RBCs/aggregate), while the others induced substantially slower agglutination and lead to smaller aggregate sizes (5-150 RBCs/aggregate). For all analyzed lectins the lectin-induced RBC agglutination of MI or AP patients was generally higher than for HV. However, only PHA induced agglutination that clearly distinguished MI from HV. Variance analysis showed that aggregate size after 15 min. agglutination induced by PHA was significantly higher in the MI group (143 RBCs/ aggregate) than in the HV (29 RBC-s/aggregate, p = 0.000). We hypothesize that pathological changes during MI induce modification of the carbohydrate composition on the RBC membrane and thus modify RBC agglutination. Occurrence of carbohydrate-lectin binding sites on RBC membranes provides evidence about MI. Due to significant difference in the rate of agglutination between MI > HV the differentiation between these groups is possible based on PHA-induced RBC-agglutination. This novel assay could serve as a rapid, cost effective valuable new tool for diagnosis of MI.

Proceedings Article | 4 March 2014 Paper

Flow cytometric assay for analysis of cytotoxic effects of potential drugs on human peripheral blood leukocytes

Kathleen Nieschke, Anja Mittag, Karolina Golab, Jozsef Bocsi, Arkadiusz Pierzchalski, Wojciech Kamysz, Attila Tarnok

Proceedings Volume 8947, 89470W (2014) https://doi.org/10.1117/12.2037741

KEYWORDS: Blood, Toxicity, Statistical analysis, Capillaries, Flow cytometry, Biological research, Organisms, Silver, Veins, Chemistry

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Proceedings Article | 9 February 2012 Paper

Comparison of quantitative flow cytometric data provided by panels with lower and increased color number

József Bocsi, Anja Mittag, Arkadiusz Pierzchalski, Adolf Baumgartner, Ingo Dähnert, Attila Tárnok

Proceedings Volume 8225, 822512 (2012) https://doi.org/10.1117/12.906712

KEYWORDS: Luminescence, Blood, Heart, Biological research, Calibration, Algorithm development, Surgery, Flow cytometry, Fluorescence resonance energy transfer, Statistical analysis

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Proceedings Article | 23 February 2011 Paper

Classification and discrimination of pediatric patients undergoing open heart surgery with and without methylprednisolone treatment by cytomics

Jozsef Bocsi, Anja Mittag, Arkadiusz Pierzchalski, Pavel Osmancik, Ingo Dähnert, Attila Tárnok

Proceedings Volume 7902, 79021O (2011) https://doi.org/10.1117/12.876487

KEYWORDS: Surgery, Heart, Blood, Statistical analysis, Modulation, Luminescence, Biological research, Flow cytometry, Cardiology, Analytical research

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Proceedings Article | 11 February 2011 Open Access

The influence of selected antimicrobial peptides on the physiology of the immune system

Karolina Golab, Anja Mittag, Arkadiusz Pierzchalski, Jozsef Bocsi, Wojciech Kamysz, Attila Tarnok

Proceedings Volume 7902, 790214 (2011) https://doi.org/10.1117/12.876485

KEYWORDS: Amplifiers, Flow cytometry, Bacteria, Physiology, Pathogens, Therapeutic agents, Defense and security, Tissues, Resistance, Antimicrobial agents

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Proceedings Article | 25 February 2010 Paper

Practical way to develop 10-color flow cytometry protocols for the clinical laboratory

Attila Tárnok, Jozsef Bocsi

Proceedings Volume 7568, 756818 (2010) https://doi.org/10.1117/12.840876

KEYWORDS: Blood, Flow cytometry, Signal detection, Luminescence, Sensors, Optical filters, Fluorescence resonance energy transfer, Biology, Diagnostics, Light sources

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Proceedings Article | 13 February 2009 Paper

Evaluation of optimal DNA staining for triggering by scanning fluorescence microscopy (SFM)

Anja Mittag, Monika Marecka, Arkadiusz Pierzchalski, Wolf Malkusch, József Bocsi, Attila Tárnok

Proceedings Volume 7182, 71820L (2009) https://doi.org/10.1117/12.808920

KEYWORDS: Luminescence, Atomic force microscopy, Signal detection, Optical filters, Statistical analysis, Microscopes, Blood, Biological research, Microscopy, Signal analyzers

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Proceedings Article | 12 February 2009 Paper

Triggering of leukocytes by phase contrast in imaging cytometry with scanning fluorescence microscope (SFM)

József Bocsi, Arkadiusz Pierzchalski, Monika Marecka, Wolf Malkusch, Attila Tárnok

Proceedings Volume 7182, 71821S (2009) https://doi.org/10.1117/12.809191

KEYWORDS: Luminescence, Signal detection, Phase contrast, Atomic force microscopy, Blood, Microscopy, Microscopes, Image analysis, Light scattering, Particles

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Proceedings Article | 12 February 2009 Paper

Evaluation of slide based cytometry (SBC) for concentration measurements of fluorescent dyes in solution

Arkadiusz Pierzchalski, Monika Marecka, Hans-Willy Müller, József Bocsi, Attila Tárnok

Proceedings Volume 7182, 71821R (2009) https://doi.org/10.1117/12.808797

KEYWORDS: Luminescence, Molecules, Imaging systems, Digital imaging, Statistical analysis, Liquids, Fluorescence spectroscopy, Silver, Light emitting diodes, Objectives

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Flow cytometers (FCM) are built for particle measurements. In principle, concentration measurement of a homogeneous solution is not possible with FCM due to the lack of a trigger signal. In contrast to FCM slide based cytometry systems could act as tools for the measurement of concentrations using volume defined cell counting chambers. These chambers enable to analyze a well defined volume. Sensovation AG (Stockach, Germany) introduced an automated imaging system that combines imaging with cytometric features analysis. Aim of this study was to apply this imaging system to quantify the fluorescent molecule concentrations. The Lumisens (Sensovation AG) slide-based technology based on fluorescence digital imaging microscopy was used. The instrument is equipped with an inverted microscope, blue and red LEDs, double band-pass filters and a high-resolution cooled 16-bit digital camera. The instrument was focussed on the bottom of 400μm deep 6 chamber slides (IBIDI GmbH, Martinsried, Germany) or flat bottom 96 well plates (Greiner Bio One GmbH, Frickenhausen, Germany). Fluorescent solutions were imaged under 90% pixel saturation in a broad concentration range (FITC: 0.0002-250 μg/ml, methylene blue (MethB): 0.0002-250 μg/ml). Exposition times were recorded. Images were analysed by the iCys (CompuCyte Corp., Cambridge, MA, USA) image analysis software with the phantom contour function. Relative fluorescence intensities were calculated from mean fluorescence intensities per phantom contours divided by the exposition time. Solution concentrations could be distinguished over a broad dynamic range of 3.5 to 5.5 decades log (range FITC: 0.0002-31.25μg/ml, MethB: 0.0076-31.25μg/ml) with a good linear relationship between dye concentration and relative fluorescence intensity. The minimal number of fluorescent molecules per pixel as determined by the mean fluorescence intensity and the molecular weight of the fluorochrome were about 800 molecules FITC and ~2.000 MethB. The novel slide-based imaging system is suitable for detection of fluorescence differences over a broad range of concentrations. This approach may lead to novel assays for measuring concentration differences in cell free solutions and cell cultures e.g. in secretion assays.

Proceedings Article | 29 February 2008 Paper

Immunological changes following protein losing enteropathy after surgery total cavopulmonary connection (TCPC) by cytomics

József Bocsi, Dominik Lenz, Anja Mittag, Ursula Sauer, Lena Wild, John Hess, Dietmar Schranz, Jörg Hambsch, Peter Schneider, Attila Tárnok

Proceedings Volume 6859, 68590N (2008) https://doi.org/10.1117/12.761640

KEYWORDS: Surgery, Proteins, Blood, Heart, Luminescence, Cardiology, Receptors, Analytical research, Biological research, Cardiovascular surgery

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Proceedings Article | 27 March 2006 Paper

Automated four color CD4/CD8 analysis of leukocytes by scanning fluorescence microscopy using Quantum dots

Jozsef Bocsi, Anja Mittag, Viktor Varga, Bela Molnar, Zsolt Tulassay, Ulrich Sack, Dominik Lenz, Attila Tarnok

Proceedings Volume 6096, 60960Z (2006) https://doi.org/10.1117/12.644544

KEYWORDS: Luminescence, Atomic force microscopy, Quantum dots, Microscopes, Blood, Biological research, Microscopy, Optical filters, Multiplexing, Analytical research

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Proceedings Article | 21 February 2006 Paper

Apoptosis of circulating lymphocytes during pediatric cardiac surgery

J. Bocsi, M. Pipek, J. Hambsch, P. Schneider, A. Tárnok

Proceedings Volume 6088, 60880J (2006) https://doi.org/10.1117/12.645021

KEYWORDS: Cell death, Surgery, Blood, Light scattering, Statistical analysis, Diagnostics, Medicine, Cardiovascular surgery, Defense and security, Flow cytometry

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Proceedings Article | 21 February 2006 Paper

Proliferation/apoptosis determination by tissue cytometry in gastrointestinal fresh frozen sections using triple labeling and automated scanning fluorescence microscopy

Jozsef Bocsi, Ferenc Sipos, Levente Ficsor, Viktor Varga, Zsolt Tulassay, Attila Tarnok, Béla Molnár

Proceedings Volume 6088, 60880I (2006) https://doi.org/10.1117/12.645823

KEYWORDS: Luminescence, Atomic force microscopy, Cell death, Tissues, Microscopy, Biopsy, Microscopes, Control systems, Biological research, Visualization

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Proceedings Article | 29 March 2005 Paper

Immunophenotyping by slide-based cytometry and by flow cytometry are comparable

Andreas Gerstner, Wiebke Laffers, Anja Mittag, Ingo Daehnert, Domnik Lenz, Friedrich Bootz, Jozsef Bocsi, Attila Tarnok

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.588644

KEYWORDS: Luminescence, Flow cytometry, Blood, Glasses, Laser scanners, Statistical analysis, Signal detection, Cardiology, Laser systems engineering, Monoclonal antibodies

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Proceedings Article | 24 March 2005 Paper

Tissomics: two- and three-dimensional distribution of nuclei in brain tissue using laser scanning cytometry (LSC)

Domnik Lenz, Anja Mittag, Birgit Mosch, Jozsef Bocsi, Thomas Arendt, Attila Tarnok

Proceedings Volume 5701, (2005) https://doi.org/10.1117/12.590695

KEYWORDS: Tissues, Brain, Luminescence, Solids, Neurons, Laser tissue interaction, Neuroimaging, Laser scanners, Argon ion lasers, Data acquisition

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Proceedings Article | 1 July 2004 Paper

Sequential photobleaching for increasing the measurable fluorochromes by laser scanning cytometry

Anja Mittag, Dominik Lenz, Jozsef Bocsi, Ulrich Sack, Andreas Gerstner, Attila Tarnok

Proceedings Volume 5322, (2004) https://doi.org/10.1117/12.528208

KEYWORDS: Luminescence, Optical filters, Blood, Laser scanners, Statistical analysis, Data acquisition, Glasses, Biological research, Fluorescence resonance energy transfer, Microscopes

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For immunophenotypic analysis more measurable parameters for the discrimination of leukocyte subsets are necessary. With a single scan six fluorochromes can be distinguished with the Laser Scanning Cytometer (LSC). Due to the number of PMTs the amount of simultaneously measurable fluorescences per scan is limited. Nevertheless, the amount of measurable colors can be improved to eight by appropriate change of the filter settings and two scans per specimen. Aim of this study was to use the special features of Slide based Cytometry (SBC) beyond filter change, remeasurement and merging to distinguish fluorochromes with similar emission spectra. The photosensitivity of fluorochromes that are excited and emit in a similar wavelength range may be very different. The number of measurable parameters per PMT was increased using photosensitivity of different fluorochromes as additional criteria. Peripheral blood leukocytes were stained with antibodies conjugated to the fluorochromes APC, APC-Cy5.5 and Alexa-Fluor 633 and mounted on conventional uncoated glass slides with Fluorescence mounting medium. Specimens were excited in the LSC with the HeNe (633nm) Laser and measured at different filter settings (670/20nm-filter for APC/ALEXA 633 and 710/20nm-filter for APC-Cy5.5). At this point, APC-Cy5.5 and APC/ALEXA633 were already distinguishable. In order to differentiate between APC and ALEXA633 photobleaching was performed by repeated excitation with the laser at 633nm. Control measurements proved that APC is much more sensitive against laser excitation, i.e. looses much more fluorescence intensity than ALEXA633. The separate measurements (before/after filter change and before/after bleaching) were merged into one file. The photostability of Alexa-Fluor 633 (1.02% bleach per scan) and APC (5.74% bleach per scan) are substantially different. Therefore, after bleaching and merging both fluorochromes can be distinguished and are regarded by the software as separate parameters. The fluorochromes APC/ALEXA633 and APC-Cy5.5 can be discriminated by changing the emission filters before bleach. By sequential photobleaching, change of filters and subsequent merging of the data the number of simultaneously measurable “colors” is substantially increased.

Proceedings Article | 1 July 2004 Paper

Assessment of DNA replication in central nervous system by Laser Scanning Cytometry

Dominik Lenz, Birgit Mosch, Jozsef Bocsi, Thomas Arendt, Attila Tárnok

Proceedings Volume 5322, (2004) https://doi.org/10.1117/12.528168

KEYWORDS: Neurons, Brain, Alzheimer's disease, Luminescence, Tissues, Laser scanners, Proteins, Data acquisition, Solids, Argon ion lasers

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Proceedings Article | 22 June 2004 Paper

Clinical and laboratory applications of slide-based cytometry with the LSC, SFM, and the iCYTE imaging cytometer instruments

Jozsef Bocsi, Ed Luther, Anja Mittag, Ingo Jensen, Ulrich Sack, Dominik Lenz, Lajos Trezl, Viktor Varga, Beea Molnar, Attila Tarnok

Proceedings Volume 5328, (2004) https://doi.org/10.1117/12.529314

KEYWORDS: Atomic force microscopy, Cell death, Luminescence, Microscopes, Microscopy, Tumors, Digital imaging, Optical filters, Signal detection, Blood

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Proceedings Article | 1 June 1999 Paper

Detection of leukocyte filtration and potential selective migration during use of cardiopulmonary bypass in cardiac surgery by flow cytometry

Joerg Hambsch, Veronika Schlykow, Jozsef Bocsi, Peter Schneider, Michal Pipek, Attila Tarnok

Proceedings Volume 3604, (1999) https://doi.org/10.1117/12.349200

KEYWORDS: Blood, Surgery, In vitro testing, In vivo imaging, Flow cytometry, Tissues, Chromium, Optical filters, Statistical analysis, Cardiology

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