Rance Solomon, James Cooper, Gabriel Welker, Elaura Aguilar, Brooke Flanagan, Chelsey Pennycuff, David Scott, Anthony Farone, Mary Farone, Daniel Erenso, Robert Mushi, Maria del Pilar Aguinaga
Genetic mutation of the β-globin gene or inheritance of this mutated gene changes the chemical composition of the oxygen-carrying hemoglobin molecule that could lead to either the heterozygote genotype, resulting in sickle cell trait (SCT), or the homozygote genotype, resulting in sickle cell anemia (SCA). These mutations could affect the reversible elastic deformations of the red blood cells (RBCs) which are vital for biological functions. We have investigated this effect by studying the differences in the deformability of RBCs from blood samples of an individual with SCT and an untreated patient with SCA along with hemoglobin quantitation of each blood sample. Infrared 1064 nm laser trap force along with drag shear force are used to induce deformation in the RBCs. Ultra2-High Performance Liquid Chromatography (UHPLC) is used for the hemoglobin quantitation.
A laser tweezer (LT) along with advanced imaging techniques has been widely applied to manipulate and study living as well as nonliving microscopic objects. In this study we present yet another novel application of LTs for a precise measurement of the viscosities of fluids in a micro-volume flow. We have demonstrated this novel application by measuring the viscosity of a fetal bovine serum (FBS) using a LT constructed from a single intensity gradient laser trap. By calibrating the LT using dielectric silica micro-beads in a fluid with a known viscosity, specifically water, and by suspending same size of silica beads in the FBS and trapping with the same trap, we have determined the viscosity of the FBS at different temperatures. We have used the relationship between the trapping and Stoke’s drag force for a constant drag speed to determine the viscosity. We have also analyzed the viscosities determined in comparison with corresponding viscosities measured using an Ostwald viscometer.
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