We propose a novel microscopy method, called chiral structured illumination microscopy, for fast imaging fluorescent chiral domains at sub-wavelength resolution. This method combines three main techniques, namely structured illumination microscopy, fluorescence-detected circular dichroism and optical chirality engineering. By generating the moir`e effect based on the circular dichroism response of the sample, chiral SIM is able to restore the high spatial frequency information on the chiral domains and reconstruct an image with super resolution. We establish the theoretical framework of chiral SIM and present a numerical demonstration that indicates its superior resolving power over that of diffraction-limited wide-field imaging methods. We also discuss the possibility of applying nanostructures that form the superchiral near fields to boost the circular dichroism response in the proposed chiral SIM method.
High-speed tracking of nano-objects is a gateway to understanding processes at the nanoscale. Here we will present our results on tracking single or ensembles of nano-objects inside optofluidic fibers and on-chip waveguides via elastic light scattering. The nano-objects diffuse inside a channel of a microstructured waveguide and the light scattered by the nano-object is detected transversely via a microscope. We will present the fundamentals of this approach and focus on selected results including 3D tracking in dual-core microstructured fibers and revealing the limits of the approach. We will also present first results on tracking inside nanoprinted on-chip waveguides.
High-speed tracking of nano-objects is a gateway to understanding biological processes at the nanoscale. Here we will present our results on tracking single or ensembles of nano-objects inside optofluidic fibers via elastic light scattering. The nano-objects diffuse inside a channel of a microstructured fiber and the light scattered by the nano-object is detected transversely via a microscope. We will present the fundamentals of this approach and focus on selected results including retrieval of the full 3D trajectory of a diffusing nano-sphere, the simultaneous detection of hundreds of nano-objects in hollow core anti-resonant fibers and first results on inactivated SARS-CoV-2.
High-speed tracking of nano-objects is a gateway to understanding biological processes at the nanoscale. Here we will present our results on tracking single or ensembles of nano-objects inside optofluidic fibers via elastic light scattering. The nano-objects diffuse inside a channel of a microstructured fiber and the light scattered by the nano-object is detected transversely via a microscope. We will present the fundamentals of this approach and focus on selected results including retrieval of the full 3D trajectory of a diffusing nano-sphere, the simultaneous detection of hundreds of nano-objects in hollow core anti-resonant fibers and first results on inactivated SARS-CoV-2.
To improve the localization accuracy and to reduce the overall photo-damage in Single Molecule Localization Microscopy (SMLM), we introduce a holography based Region-of-Interest (ROI) illumination with oblique angles. The ROI illumination is realized in two different application modes which offer different advantages. Both modes (A,B) are implemented within the same setup by modifying the illumination light using a phase-only spatial light modulator (SLM) twice. This allows to adaptively modify the size and the (excitation) angle of the required ROI illumination, resulting in reduced out-of focus signal and less overall phototoxicity. Mode A generates a nearly speckle free, circular ROI which can be obtained instantly (no iterative algorithm needed). Mode B allows to realize an arbitrarily shaped ROI but comes at the cost of a higher presence of speckle structures as well as in an increased calculation time of the holograms. In both cases illumination angles up to 60° with high NA objectives are realizable, enabling the effective selection of specific parts of a biological probe to be illuminated.
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