In a surgical pathology setting, the clinical study of tissue specimens is often limited to evaluating an effectively 2D representation of an inherently 3D specimen and disease, most commonly by a several-micron thick hematoxylin and eosin (H&E) stained glass slide. X-ray transmission allows for the study of thicker tissue volumes but does not provide soft tissue contrast. Previous studies using x-ray diffraction (XRD) have shown that XRD can differentiate some soft tissue and disease types from one another. We focus here on simulation-based trade studies using a toy model to optimize and evaluate the imaging performance of a 3D structured illumination XRD imaging scheme. In particular, we quantify the lateral and axial spatial resolution and evaluate how these parameters depend on the angular extent and beamlet configuration of the primary structured illumination beam. We observe an optimal beamlet configuration and show that a transverse resolution of 100um and an axial resolution of 500um is achievable.
This work evaluates two different detector technologies in terms of their performance in making fast, low-signal diffraction measurements. The first detector is a large-area mammography detector that uses a complementary metal-oxide semiconductor (CMOS) crystal, and the second is a cadmium-telluride photon-counting detector. By measuring the diffraction spectra for a diverse range of materials and with acquisition times ranging from 10 seconds and 0.1 seconds, we show how each detector performs as signal-to-noise ratios decrease and counting statistics become less significant. As a result, we show that the photon counting detector slightly better preserves the long-time average signal in short acquisition times in comparison to the CMOS detector when diffraction signals display sharp/narrow features, but that the detectors performed similarly for materials with much broader diffraction signals, like those associated with soft tissue and biological specimen. This leads us to conclude that the photon-counting detector is slightly higher-performing for our purposes.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.