Elucidating the neuropathophysiology of COVID-19 using quantum dot biomimetics of SARS-CoV-2

Wesley Chiang; Jennifer Urban; Angela Litzburg; Bradley Nilsson; Harris Gelbard; Todd Krauss

doi:10.1117/12.2609118

3 March 2022 Elucidating the neuropathophysiology of COVID-19 using quantum dot biomimetics of SARS-CoV-2

Wesley Chiang, Jennifer Urban, Angela Litzburg, Bradley Nilsson, Harris Gelbard, Todd Krauss

Proceedings Volume 11977, Colloidal Nanoparticles for Biomedical Applications XVII; 1197702 (2022) https://doi.org/10.1117/12.2609118
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Quantum dots were encapsulated in polymeric phospholipid micelles conjugated to multiple ligands of SARS-CoV-2 spike protein to form fluorescent biomimetic nanoparticles for SARS-CoV-2 (COVID-QDs). Phosphatidylethanolaminepolyethylene glycol (PE:PEG) was appended with bis(4-methylphenyl)sulfone to form PE:PEG:bis-sulfone and selfassembled into micelles around CdSe/CdS core/shell quantum dots via thin-film rehydration. The introduction of the bissulfone group the surface of the micelle-encapsulated quantum dots provides multiple sites for conjugation to his-tagged SARS-CoV-2 spike protein via a bisalkylation mechanism. Based on the eluted unconjugated fraction, we estimate that an average of seven spike proteins are conjugated per COVID-QD. We treated an in-vitro model system for the neurovascular unit (NVU) with these COVID-QD constructs to investigate the COVID-QDs, and by proxy SARS-CoV-2, may modulate the NVU leading to the COVID-19 associated neuropathophysiology.

Conference Presentation

Citation Download Citation

Wesley Chiang, Jennifer Urban, Angela Litzburg, Bradley Nilsson, Harris Gelbard, and Todd Krauss "Elucidating the neuropathophysiology of COVID-19 using quantum dot biomimetics of SARS-CoV-2", Proc. SPIE 11977, Colloidal Nanoparticles for Biomedical Applications XVII, 1197702 (3 March 2022); https://doi.org/10.1117/12.2609118

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