Noninvasive fluorescence and Raman spectroscopic analysis of laser welded aorta and skin tissue

Alvin Katz; Tapan K. Gayen M.D.; Glenn Minko; Alexandra Alimova; Howard E. Savage; Steven A. McCormick; Robert R. Alfano

doi:10.1117/12.465228

1 May 2002 Noninvasive fluorescence and Raman spectroscopic analysis of laser welded aorta and skin tissue

Alvin Katz, Tapan K. Gayen M.D., Glenn Minko, Alexandra Alimova, Howard E. Savage, Steven A. McCormick, Robert R. Alfano

Author Affiliations +

Proceedings Volume 4613, Optical Biopsy IV; (2002) https://doi.org/10.1117/12.465228
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Laser tissue welding involves the denaturing and partial renaturing of collagen and elastin. Tissues welded with NIR lasers tuned to the 1455 nm water absorption band have demonstrated high tensile strength with minimal collateral damage. To better understand the welding process, welded tissue samples were investigated using fluorescence imaging and Raman spectroscopy. As part of this study, human aorta, and porcine aorta and skin, specimens were investigated. Emission and excitation/emission wavelengths corresponding to tryptophan and collagen emission and slightly weaker emission for wavelengths corresponding to elastin emission. The inner surface an cross-section images of the aortic specimens exhibited a very high degree of uniformity with no indication of the presence of a weld. The Raman spectra from the aortic specimens at the weld site and a few mm away form the weld were very similar. This work indicates the emission and Raman properties of the collagen helix after welding are very similar to native collagen tissue.

Citation Download Citation

Alvin Katz, Tapan K. Gayen M.D., Glenn Minko, Alexandra Alimova, Howard E. Savage, Steven A. McCormick, and Robert R. Alfano "Noninvasive fluorescence and Raman spectroscopic analysis of laser welded aorta and skin tissue", Proc. SPIE 4613, Optical Biopsy IV, (1 May 2002); https://doi.org/10.1117/12.465228

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