Combined laser speckle imaging and fluorescent intravital microscopy for monitoring acute vascular permeability reaction

Vyacheslav Kalchenko; Igor Meglinski; Anton Sdobnov; Yuri Kuznetsov; Alon Harmelin

doi:10.1117/1.JBO.24.6.060501

31 May 2019 Combined laser speckle imaging and fluorescent intravital microscopy for monitoring acute vascular permeability reaction

Vyacheslav Kalchenko, Igor Meglinski, Anton Sdobnov, Yuri Kuznetsov, Alon Harmelin

Author Affiliations +

Funded by: Moscow Engineering Physics Institute, MEPhi Academic Excellence Project, Finnish Cultural Foundation

Journal of Biomedical Optics, Vol. 24, Issue 6, 060501 (May 2019). https://doi.org/10.1117/1.JBO.24.6.060501

Abstract

Optical clearing agents (OCAs) and many chemicals are widely used in functional diagnosis of skin tissues. Numerous studies are associated with the transcutaneous diffusion of OCA in epidermal, dermal, and hypodermal tissues, which results in changing their optical properties. In addition, an objective approach that is suitable for screening the influence of utilized OCA, as well as various chemical agents, synthetics, and nanomaterials, on blood and lymph flows is highly desirable. In our study, a highly sensitive laser speckle imaging (LSI) system and fluorescent intravital microscopy (FIM) were used team-wise to inspect the acute skin vascular permeability reaction in mouse ear during the local application of OCA on the skin surface. Fluorescent contrast material administrated intravenously was used for quantitatively assessing the intensity of vascular permeability reaction and the strength of skin irritation. The obtained results suggest that a combined use of LSI and FIM is highly effective for monitoring the cutaneous vascular permeability reaction, with great potential for assessment of allergic reactions of skin in response to interactions with chemical substances.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Vyacheslav Kalchenko, Igor Meglinski, Anton Sdobnov, Yuri Kuznetsov, and Alon Harmelin "Combined laser speckle imaging and fluorescent intravital microscopy for monitoring acute vascular permeability reaction," Journal of Biomedical Optics 24(6), 060501 (31 May 2019). https://doi.org/10.1117/1.JBO.24.6.060501

Received: 23 January 2019; Accepted: 11 April 2019; Published: 31 May 2019

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