A Mueller matrix model of biological tissues optical anisotropy is proposed. The relationships between the histological sections phase (birefringence) anisotropy parameters and polarization maps are determined. The experimental results of the brain and liver native histological sections polarization mapping of those who died with different durations of blunt mechanical trauma are presented. As part of the statistical analysis of the set of native samples digital microscopic images ellipticity coordinate distributions. The main time scenarios for changes in the biological tissues optically anisotropic architectonics polarization map’s topographic structure were determined. On this basis, a polarization method has been proposed for determining the duration of blunt mechanical trauma. The possibility of detecting, using statistical polarization markers, the duration of a mechanical injury for 18 hours with an accuracy of 45 to 60 minutes has been demonstrated. Prospects for further research using laser-induced autofluorescence polarimetry methods that are more sensitive at the molecular level are presented.
Proposed and substantiated a method for 3d Mueller-matrix reproduction of the distributions of parameters of linear and circular birefringence and dichroism of partially depolarizing polymer films in publishing and printing business.
This work is aimed at generalizing the methods of laser polarimetry in the case of partially depolarizing optically anisotropic polymer layers. A method of differential Mueller-matrix mapping was proposed and substantiated for reproducing the distributions of the parameters of linear and circular birefringence and dichroism of methyl acrylate layers under various temperature conditions (200 ‒ group 1) and (450 ‒ group 2).
The article presents the results of algorithmic approbation of digital histological research methods of the age of damage to the tissues of the brain, liver and kidney, as well as the myocardium and lung tissue on the basis of polarization reconstruction of linear birefringence maps of protein fluorophores of fibrillar networks; determining the relationship between the temporal change in the magnitude of the statistical moments of the 1st - 4th orders, characterizing the distribution of the magnitude of the degree of crystallization of histological sections of the brain, liver and kidney, as well as the myocardium and lung tissue, and the duration of damage; establishment of time intervals and accuracy of determining the duration of damage to human internal organs by digital histological methods of polarization reconstruction (tomography) the degree of crystallization of samples of histological sections of the brain, liver and kidney, as well as myocardium and lung tissue.
KEYWORDS: Scattering, Light scattering, Proteins, Bone, Particles, Spherical lenses, Polarization, Macromolecules, Temperature metrology, Chemical elements
The article presents the results of a study of the regularities of the scattering of polarized light by fish mucus and fish scales in order to identify matrix elements that are most sensitive to changes in the conformation, shape and size of protein macromolecules of fish mucus, and to identify the structural features of fish scales.
Various aspects of pathology of the pancreas (PP), functional insufficiency of which causes the development of hyperglycemia, insulin resistance, metabolic syndrome, and type I and type II diabetes mellitus have attracted considerable attention among researchers. Among the exogenous influences that can have a negative effect on metabolic processes in this organ, an imbalance of macronutrients in the diet and drug-induced organ injury due to uncontrolled consumption of commonly available antipyretic analgesics, of which paracetamol (acetaminophen) is the most common are of great importance [1].
A model Jones-matrix description of the polycrystalline structure of polymeric polyethylene films (PPF) is presented. Algorithms for Jones-matrix reproduction of parameters of linear and circular birefringence of PPF are obtained. The presented results of the study of the relationship between layered maps of the elements of the Jones matrix and layered PPF maps. The values and ranges of changes in the statistical moments of the 1st - 2nd orders, which characterize the layered maps of linear and circular birefringence PPF, have been determined. Physically substantiated and experimentally determined the effectiveness of the method of 3D layer-by-layer Jones of matrix mapping PPF in differentiating their structure
Algorithms for reconstruction of linear and circular birefringence of optically thin anisotropic акрилового скла layers are presented. The technique of Jones-matrix tomography of polycrystalline acrylic glass layers has been experimentally tested. The coordinate distributions of phase anisotropy of acrylic glass samples with varying degrees of mechanical stress are determined and statistically analyzed. Criteria (statistical moments of 3rd and 4th orders) of differential diagnostics of the presence of mechanical stress were determined.
Insufficient intake of exogenous proteins under the conditions of irrational nutrition, deficiency of essential amino acids as well as increased loss of endogenous proteins with a characteristic negative nitrogen balance lead to the development of acute or chronic liver diseases. At the same time, the public availability of a wide range of drugs creates the problem of their unsystematic and irrational use for the correction of pathological conditions without proper compliance with the dosage and interval between doses. The consequence of uncontrolled self-medication is increasingly becoming drug induced liver injury.
The article contains the results of experimental testing of methods of azimuthal-invariant Mueller-matrix microscopy (Mueller-matrix invariants - MMI) of optically anisotropic fluorophores of samples of histological sections of the brain, liver and kidney, as well as myocardium and lung tissue; temporal detection of variations in the magnitude of the statistical moments of the 1st - 4th orders, characterizing the distributions of the MMI value of linear birefringence and optical activity of samples of histological sections of the brain, liver and kidney, as well as myocardium and lung tissue with different age of damage; determination of the diagnostic efficiency (time interval and accuracy) of establishing the age of damage to human internal organs by digital histological methods of MMI mapping of optical anisotropy of fluorophores in histological sections of the brain, liver and kidney, as well as myocardium and lung tissue.
The paper presents a structural and logical diagram and an analytical description of the differential diagnosis of aseptic and septic loosening of the cup of an artificial hip joint endoprosthesis using spectral-selective laser autofluorescence microscopy of coordinate distribution maps (MIF) and correlation (MCF) fluorescence intensity of polycrystalline films of synovial fluid (SF). The results of the statistical analysis [1-5] of the distributions of the MIF and MCF values of polycrystalline SF films of patients from the control group and groups with different severity of pathology of the hip joint are presented. The sensitivity, specificity and accuracy [6-9] of the method of spectral-selective laser autofluorescence microscopy MIF and MCF of the fluorescence intensity of polycrystalline films of hip joint CF have been established.
A new digital technique of objective differential diagnosis of the septic process severity was developed and tested experimentally by polarization-phase mapping of microscopic images of histological sections of the liver of laboratory rats based on statistical and information analysis of phase shift distributions.
The results of a statistical analysis of the distributions of characteristic values of the Stokes vector fourth parameter (crystallization parameter - CP) of the digital vector-parametric microscopic image of healthy spleen and with spleen with sepsis histological sections are presented. We determined the statistical significance of differentiation of vector-parametric maps of the microscopic image of histological sections of rat spleens from control group 1 and research groups 2-4 with different severity of septic pathology. The most diagnostic-sensitive statistical criteria for differentiating vector-parametric maps of microscopic images of histological sections of the spleen are established.
In this paper are presented: structural-logical diagram and design of a phasemetric study of microscopic images of blood films of laboratory rats; model analysis of the polycrystalline structure of blood films of laboratory rats; structurally logical diagram and characteristics of the optical arrangement of the system of phasemetric mapping of microscopic images of blood films of laboratory rats; algorithms for statistical processing of experimental data of a system for phasemetric mapping of microscopic images of blood films of laboratory rats; information analysis algorithms to determine the strength of the diagnostic method of phasemetric mapping of microscopic images of blood films of laboratory rats by establishing a set of operational characteristics - sensitivity, specificity and accuracy.
For differential diagnosis of severity of the septic process, the method of mapping vector-parametric microscopic images of the polycrystalline component of histological sections of the internal organs of rats was used. A statistical analysis of the distribution of the number of characteristic values of the Stokes vector of polarizing microscopic images of histological sections of the internal organs of laboratory rats was carried out. The relationships between the values of statistical moments of the 1st - 2nd orders of magnitude characterizing the distribution of the number of characteristic values of the Stokes vector of polarizing microscopic images of histological sections of the internal organs of laboratory rats and the severity of the septic process are determined. Quantitative parameters that are most sensitive to septic conditions have been established, which ensure the implementation of statistically confidance differentiation of histological sections of the internal organs of laboratory rats. From the standpoint of evidence-based medicine, the operational characteristics of the diagnostic forces of the vector-parametric microscopy method are established with an estimate of the number of characteristic values of the fourth Stokes vector of polarization images of histological sections of the internal organs of laboratory rats.
The method of stock-polarimetry with spatial-frequency filtering of the distributions of the Muller-matrix invariants (MMI) for analytically differentiating the manifestations of the phase anisotropy mechanisms of different-scale fibrillar networks of histological sections of biological tissues of different morphological structure and physiological state is analytically substantiated. It has been established that all statistical moments of the first and fourth orders characterizing the distribution of the values of the matrix element of the linear birefringence of the large-scale fibrillar optically anisotropic component of benign and malignant tumors are diagnostically sensitive. The following levels of balanced accuracy have been achieved: 77,8% Ri=3;4 (good quality diagnostic test), Ri=1;2 = 91,7% 94,4% (excellent quality diagnostic test). For the first time, a polarization reconstruction method with spatial-frequency differentiation of the distributions of MMI values characterizing circular birefringence of histological sections of multilayer biological tissues was developed to differentiate changes in the phase anisotropy of polycrystalline networks in the early stages of oncological pathology - precancerous conditions (simple atrophy, polyp) of the rectal wall. On this basis, a high level of balanced accuracy R ~ 85% of the diagnostic test for differentiating the manifestations of the optical activity of endometrial networks in a precancerous state has been achieved.
This section contains the results of a study of the relationships between the 3D distributions of the optical anisotropy parameters of polycrystalline networks of biological fluid films of different biochemical composition and the layered phase sections of volume distributions of the magnitude and parameter phase of the "two-point" Stokes vector of the microscopic image.
The use of physically sound and analytically determined algorithms for reconstructing parameters characterizing linear birefringence and dichroism of networks of biological crystals in differentiating changes in optical anisotropy associated with different degrees of severity of pathology - precancerous (atrophy and polyp endometrium) conditions of the cervix. Development and testing of the ―two-wave‖ method of the Muller-matrix reconstruction of parameter values characterizing the phase and amplitude anisotropy of polycrystalline films of bile and blood plasma for the diagnosis of systemic (type II diabetes) and oncological (breast cancer) diseases.
This manuscript presents materials on the study of the spectral dependences of the parameters of the Stokes vector of electromagnetic radiation transmitted through layers of synovial fluid of the knee joint with various types of pathology.The spectral coefficients of Gram - Charlier decomposition of spectral Stokes - polarimetric dependences are established and the sensitivity and specificity of this method for the diagnosis or differentiation of the type of knee joint pathology are determined.
The given data on the optical arrangement, in which the coordinate distributions of the real and imaginary component of the elements of the Jones matrix of optically thin polycrystalline layers are determined. Algorithms are presented and an experimental method for measuring the real and imaginary component of Jones-matrix images is analyzed. The experimental results of the study of statistical, correlation, and fractal parameters, which characterize the real component of the Jones-matrix image of polycrystalline networks of flat layers of the main types of human amino acids, are presented.
The presented report contains the following materials and results: 1. The structural-logical scheme and design of studies using polarization-phase tomography methods of linear and circular birefringence and dichroism of the polycrystalline structure of histological sections of the brain. 2. Differential diagnosis of the formation of hemorrhages of traumatic origin, ischemic cerebral infarction and hemorrhagic genesis by differential Mueller-matrix mapping of phase anisotropy: • maps of circular birefringence (PCB) of brain histological sections and operational characteristics of the method of their statistical analysis; • maps of linear birefringence (PLB) of histological sections of the brain and operational characteristics of the method of their statistical analysis.
Determination of praline concentration in samples of natural waters, based on the measurement value angle of the plane of polarization of radiation of a certain wavelength λ, which has been studied by the liquid thickness l = 5 sm was developed. Three wavelength λ = 400, 450, 500 nm were used. A series of reference samples was produced: aqueous solutions of L and D forms proline of a known concentration within c = (0.001 – 0,1) %. Based on these studies, for each λ gauge dependent quantities of angle α = f (c) was built. Using them and conducting measurements of angles α for the real natural environments, the possibility of determination of the concentration of proline in them was actualized. It was determined that in waters within the city of Chernivtsy and its surroundings occured proline of two forms: L and D, the concentration of which is within c = (0,005 - 0,094)%
The given data on the optical arrangement, in which the coordinate distributions of the real and imaginary component of the elements of the Jones matrix of optically thin polycrystalline layers are determined. Algorithms are presented and an experimental method for measuring the real and imaginary component of Jones-matrix images is analyzed. The experimental results of the study of statistical, correlation, and fractal parameters, which characterize the real component of the Jones-matrix image of polycrystalline networks of flat layers of the main types of human amino acids, are presented.
The results of statistical dependence and correlation structures of two-dimensional Mueller matrix elements in various spectral regions of laser radiation by changes in the distribution of orientations of optical axes and birefringence of protein crystals. Namely, a two-wave (“red-blue”) approach – layer of biological tissues irradiated by He-Ne laser (λ1 = 0,63μm ) and He-Cd laser (λ1=0,41μm )was used Conducted analysis of polarimetric sensitivity was made, a state of polarization points that contain volumetric structures of biological objects to spectral region of laser radiation was detected.
Presented research materials: • coordinate maps of the degree of depolarization (CMD) of polycrystalline structures of histological sections of parenchymal (spleen) biological tissues of the deceased with different levels of blood loss; • magnitudes and ranges of changes in the statistical moments of the 1st - 4th orders characterizing the distribution of the magnitude of the CMD of biological organs (spleen, kidney) of the deceased with varying degrees of blood loss; • efficiency and accuracy of determining the degree of blood loss of the deceased by the method of diffuse Mueller-matrix polarimetry of polycrystalline structures of histological sections of parenchymal tissues of the deceased with different levels of blood loss.
This report contains theoretical algorithm for the differential representation of a phase-inhomogeneous biological object as a set of consecutively located optically anisotropic layers; theoretical algorithms for the decomposition of the Mueller-matrix of the diffuse biological layer in the basis of differential matrices of the 1st and 2nd orders; analytical relations for determining the magnitude of the set of elements of differential matrices of the 1st and 2nd orders;information and characteristics of optical schemes of experimental devices.
In this paper are presented next research materials: • structural-logical scheme for diagnosing the limitation of death (LD) by the methods of polarizing microscopic tomography of the polycrystalline structure of vitreous preparations; • the temporal dynamics of necrotic changes in the coordinate distributions of the magnitude of the linear birefringence (LB) of the crystalline fraction of VB layers of the deceased; • the magnitudes and ranges of the temporal variation of the statistical moments of the 1st to the 4th orders characterizing the distributions of the LB value of the crystal fraction of the VB layers of the deceased; • efficiency and accuracy of LD determination by the method of polarization microscopic tomography LB polycrystalline component of the VB layers of the deceased; • the effectiveness of the use of wavelet analysis of the coordinate distributions of the LB value of a polycrystalline fraction of VB layers of the deceased in certain LD.
The results of Jones matrix mapping of optically thin, non-depolarizing polycrystalline films of synovial fluid (of human joint at reactive and aseptic synovitis) and urine (of healthy donors – patients with albuminuria) are presented. The Jones-matrix model of polarization manifestations of phase anisotropy mechanisms is presented. The method of experimental measurement of coordinate distributions of the values of the modulus and the phase of the Jones matrix elements is suggested. In the framework of statistical and cross-correlation approaches, the maps of the modulus and phase of the Jones-matrix images of optically thin polycrystalline films of human organs with different pathologies are analyzed. A set of objective parameters (statistical and generalized correlation moments) of differential diagnostics of the reactive and aseptic synovitis of the knee joint, as well as of albuminuria was determined with excellent and good balanced precision.
The paper presents the results of Jones-matrix mapping of optically thin, non-depolarizing biological fluids with different polycrystalline structure – bile and cerebrospinal fluid (liquor). The Jones matrix model of manifestations of polarizing mechanisms of phase anisotropy of such layers is presented. The technique of experimental measurement of coordinate distributions of the modulus and phase values of Jones matrix elements is suggested. Within the statistical and cross-correlation approaches the modulus and phase maps of Jones matrix images of optically thin layers of polycrystalline films of plasma and cerebrospinal fluid are analyzed. A set of objective parameters (statistical and generalized correlation moments), which are the most sensitive to changes in the phase of anisotropy, associated with the features of polycrystalline structure of films of biological fluids of various organs are determined.
An optical-physical system for extracting information about the fluctuations in the optical anisotropy of strongly scattering biological tissues is considered. A model is proposed for the formation of a depolarized background by birefringent and dichroic structures. An explicit form and symmetry of the completely depolarized component of the Mueller matrix is determined, a second-order differential matrix. An algorithm for the analytic determination of the distributions of elements of a second-order differential matrix is found. Interrelations between the magnitude of the elements of the second-order differential matrix and the fluctuations of the linear and circular birefringence-dichroism are obtained. The technique of diffuse tomography of an optically anisotropic component of strongly scattering biological tissues has been developed and experimentally tested. Maps of the distributions of the elements of the completely depolarized component of the Mueller matrix of the histological sections of the internal organs of the healthy and of the diabetic rats . The sensitivity, specificity and balanced accuracy of the method of diffuse tomography of the polycrystalline structure of tumors of the uterine wall and the degree of hemorrhage of the liver are determined.
A model of multilayer polycrystalline structure of films of biological fluids of human organs has been developed. Each layer is associated with a partial Jones-matrix operator of phase and amplitude anisotropy. A new principle for detecting polarization-inhomogeneous object fields using a coherent laser wave is proposed. Algorithms for digital holographic reconstruction of field distributions of complex amplitudes in the plane of a polycrystalline film of a biological fluid are found. A new optical technique is proposed: direct measurement of 3D distributions of elements of the Jones matrix. Maps of layer-by-layer distributions of elements of the Jones matrix of polycrystalline urine films are studied. Sensitivity, specificity and balanced accuracy of the 3D Jones-matrix tomography method of the polycrystalline structure of urine films of healthy donors and patients with albuminuria were determined. Within the framework of the statistical analysis of stratified maps of elements of the Jones matrix of polycrystalline urine films, objective criteria for the early diagnosis of the onset and course of albuminuria were found.
The results of statistical dependence and correlation structures of two-dimensional Mueller matrix elements in various spectral regions of laser radiation by changes in the distribution of orientations of optical axes and birefringence of protein crystals. Namely, a two-wave (“red-blue”) approach – layer of biological tissues irradiated by He-Ne laser (λ1 = 0,63μm ) and He-Cd laser (λ1 = 0,41μm )was used Conducted analysis of polarimetric sensitivity was made, a state of polarization points that contain volumetric structures of biological objects to spectral region of laser radiation was detected.
The paper presents the results of Jones-matrix mapping of uterine wall histological sections with second-degree and third-degree endometriosis. The technique of experimental measurement of coordinate distributions of the modulus and phase values of Jones matrix elements is suggested. Within the statistical and cross-correlation approaches the modulus and phase maps of Jones matrix images of optically thin biological layers of polycrystalline films of plasma and cerebrospinal fluid are analyzed. A set of objective parameters (statistical and generalized correlation moments), which are the most sensitive to changes in the phase of anisotropy, associated with the features of polycrystalline structure of uterine wall histological sections with second-degree and third-degree endometriosis are determined.
It has been proposed the optical model of Jones-matrix description of mechanisms of optical anisotropy of polycrystalline films of human bile, namely optical activity and birefringence. The algorithm of reconstruction of distributions of parameters - optical rotation angles and phase shifts of the indicated anisotropy types has been elaborated. The objective criteria of differentiation of bile films taken from healthy donors and patients with cholelithiasis by means of statistic analysis of such distributions have been determined. The operational characteristics (sensitivity, specificity and accuracy) of Jones-matrix reconstruction method of optical anisotropy parameters were defined.
A new azimuthally stable polarimetric method for processing of microscopic images of optically anisotropic structures of different biological layers histological sections is proposed. A new model of phase anisotropy definition of biological tissues by using superposition of Mueller matrices of linear birefringence and optical activity is proposed. The matrix element M44 has been chosen as the main information parameter, which value is independent of rotation angle of both sample and probing beam polarization plane.
A new information optical technique of diagnostics of the structure of polycrystalline films of bile is
proposed. The model of Mueller-matrix description of mechanisms of optical anisotropy of such objects as
optical activity, birefringence, as well as linear and circular dichroism is suggested. The ensemble of
informationally topical azimuthally stable Mueller-matrix invariants is determined. Within the statistical
analysis of such parameters distributions the objective criteria of differentiation of films of bile taken from
healthy donors and diabetes of type 2 were determined. From the point of view of probative medicine the
operational characteristics (sensitivity, specificity and accuracy) of the information-optical method of
Mueller-matrix mapping of polycrystalline films of bile were found and its efficiency in diagnostics of
diabetes extent of type 2 was demonstrated. Considered prospects of applying this method in the diagnosis
of cirrhosis.
This research presents the results of investigation of laser polarization fluorescence of biological layers
(histological sections of the myocardium). The polarized structure of autofluorescence imaging layers of biological
tissues was detected and investigated. Proposed the model of describing the formation of polarization inhomogeneous of
autofluorescence imaging biological optically anisotropic layers. On this basis, analytically and experimentally tested to
justify the method of laser polarimetry autofluorescent. Analyzed the effectiveness of this method in the postmortem
diagnosis of infarction. The objective criteria (statistical moments) of differentiation of autofluorescent images of
histological sections myocardium were defined. The operational characteristics (sensitivity, specificity, accuracy) of
these technique were determined.
Our research is aimed at designing an experimental method of Fourier’s laser polarization phasometry of the layers of human effusion for an express diagnostics during surgery and a differentiation of the degree of severity (acute - gangrenous) appendectomy by means of statistical, correlation and fractal analysis of the coherent scattered field. A model of generalized optical anisotropy of polycrystal networks of albumin and globulin of the effusion of appendicitis has been suggested and the method of Fourier’s phasometry of linear (a phase shift between the orthogonal components of the laser wave amplitude) and circular (the angle of rotation of the polarization plane) birefringence with a spatial-frequency selection of the coordinate distributions for the differentiation of acute and gangrenous conditions have been analytically substantiated. Comparative studies of the efficacy of the methods of direct mapping of phase distributions and Fourier’s phasometry of a laser radiation field transformed by the dendritic and spherolitic networks of albumin and globulin of the layers of effusion of appendicitis on the basis of complex statistical, correlation and fractal analysis of the structure of phase maps.
This research is aimed to investigate the reliability of Mueller-matrix differentiation of birefringence
change of optically thick layers of biological tissues at the early stages of the change in their physiological
state. This is performed by measuring the set of asymmetry and excess values of Mueller matrix image of the
phase element M44 in various points of the object under investigation.
The model of laser polarization fluorescence of biological tissues considering the mechanisms of
optically anisotropic absorption – linear and circular dichroism of protein networks was suggested.Muellermatrix
rotation invariants characterizing polarization manifestations of laser fluorescence are determined.The
interconnections between the statistical, correlation and fractal parameters characterizing the Mueller-matrix
images of laser polarization fluorescence and the peculiarities of the mechanisms of optically anisotropic
absorption of histological sections of uterus wall biopsy were found. Effectiveness of the method of azimuthinvariant
Mueller-matrix mapping of laser polarization fluorescence of protein networks in the task of
differentiation of benign and malignant tumors of uterus wall was demonstrated.
The results of investigating the absorption spectra of blood plasma in the visible and infrared parts of spectra obtained
using the techniques of spherical photometer and spectrophotometric complex "Specord IR75" are presented. The
possibility of using these spectra for diagnoses the cases of diffuse toxic goiter and nodular goiter and control of
treatment process in postsurgical period in the cases of thyroid gland surgery is estimated.
The results of absorption spectra of blood plasma in the ultraviolet and visible areas of the spectrum using the technique
of spherical photometer. Possibilities of using these spectra to detect the diseases - diffuse toxic goiter and nodular
euthyroid goiter and to control the surgical treatment of this pathology.
Is offered a modificated technology for photodiodes based on n+-n-GaP-SnO2(F). This technology is based on analysis
of parameters, which are determining the sensitivity of the photodiode with the surface-barrier structure. Also, a special
algorithm of computer simulation was developed.. As a result a monochromatic current sensitivity at a wavelength 254
nm (no more than 0,09 - 0,08 A/W) was obtained. The uniformity of the sensitivity of a photosensitive element was no
more than 5%.
A method for determining optical proline concentrations in samples of natural waters, based on the
measurement value angle of the plane of polarization of radiation of a certain wavelength λ, which has been
studied by the liquid thickness l = 5 sm was developed.
Three wavelength λ = 400, 450, 500 nm were used. A series of reference samples was produced:
aqueous solutions of L and D forms proline of a known concentration within c = (0.001 - 0,1) %.
Based on these studies, for each λ gauge dependent quantities of angle α = f (c) was built. Using them
and conducting measurements of angles α for the real natural environments, the possibility of determination
of the concentration of proline in them was actualized.
It was determined that in waters within the city of Chernivtsy and its surroundings occured proline of
two forms: L and D, the concentration of which is within c = (0,005 - 0,094)%.
There have been given the results of the spectrophotometric examination of the dogs' and rats' venous and whole blood
plasma taken in the process of the peritonitis progressing within the spectral interval λ = 220 - 320 nm (for plasma)
and λ = 350 - 610 nm (for the whole blood). It has been defined that D-optical density values in the field of the long-waved
maximum of plasma absorption intensity of the venous blood at λ = 280 nm depend upon the intensity of the
inflammatory process and also upon the circumstances against the background of which it started to progress. It was
found out that the dynamics of D= values changes for λ = 540 (or 570) nm in the process of the peritonitis progressing
in case of the whole blood taken from a portal vein is a mirror symmetrical if to compare to the same dynamics for the
blood from cava inferior. The defined conformities with regularities may have a diagnostic meaning. It was also found
out that the biggest influence upon the dynamics of D-values at λ = 280nm of the venous blood plasma has the content of
the circulating immune complexes, necrosis factor of α-tumors and interleukin - 2, the changes of which explain for
almost on 100% the distribution of the optical density parameters and what proves a possible immunologic explanation
of its changes.
There have been given the results of the investigating the reflection coefficients of some plants' leaves with very smooth
surfaces in the polarized light for those wave lengths for which the inner composition in the reflected emission does not
practically exist. The optical constants for the mentioned objects of investigation have been defined, also Fresnel
formulas as well as the method of the determination of the optical constants based on the measuring the reflection
coefficients of the polarized emission for the definite fixed angles of incidence.
It is set that there are two maximums in UF- region absorption of vein blood plasma of a man: at λ = 235 nm and at λ =
280 nm. It is shown that there are the substantial changes of values of the optical density D comparative with controls
(for donors) exactly in a maximum at development of sharp surgical diseases of organs of abdominal region λ = 280 nm,
in that time as maximum at λ = 235 nm in this plan is not informing. Resulted results of researches of dynamics of
changes of optical properties of vein blood plasma in UF- region of patients with pathology of abdominal region organs
in after operating period (sharp appendicitis, sharp pancreatitis, intestinal impassability and others like that), which can
have the diagnostic value.
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