In this study, we developed an improved piston-based specimen holder to provide even pressure distribution across an irregular tissue surface. A series of support fixtures are also developed to facilitate the pressure distribution from the piston to image specimens with small contact area relative to thickness such as bisected shave skin biopsies. Using this capability, we demonstrate imaging of tall and narrow biopsy specimens with precise coregistration to conventional histology as well as rapid imaging of Mohs margins during surgery.
We developed a rapid tissue clearing technique combined with two-photon fluorescence microscopy (cFTPM) to image melanoma in situ biopsy volumetrically. cFTPM can provide rapid volumetric melanoma in situ biopsy images with the potential to provide same-day melanoma biopsy turnaround time with full volumetric images. The overall time span of our process, including tissue clearing and imaging, is faster than conventional histology processing.
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