We present a novel technique that uses hybrid digital holography to observe three-dimensional morphological changes in cardiac organoids and measure heart rates simultaneously. By reconstructing the real-time spatial topology, we capture dynamic morphological changes in three-dimensional cardiac organoids, especially in the central region where traditional optical imaging methods are limited. We analyze the correlation between the phase analysis results of digital holography and the corresponding electronic signal amplitude with heartbeat data. Fluorescent modes can also be used to investigate heart rate, ROS activation, and organic cell activation. In addition, dark field mode allows you to simultaneously evaluate the distribution of nanoparticles about 100 nm in size on the heart surface and their effect on the heart rate. Our work demonstrates the potential of hybrid digital holography as an innovative optical analysis method in heart and tissue analysis, providing valuable insights into the relationship between cardiac tissue dynamics and heart rate
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