Spectral phasors are currently based on fluorescence spectra in the wavelength domain. However, this is a problem when using spectral phasors to quantify emitters since wavelength represented spectra are not proportional to emitter population. Here we show the effect of unmixing using spectra in their transition dipole moment representation for a theoretical two component system and on a real seven component system.
The performance of industrial ultrafast lasers with average power ranging from 10-1000 Watts depends on their wavelength, pulse duration, total power, and repetition rate. However, variability in laser performance, despite similar characteristics and manufacturing, indicates the current metrics are not enough. Here we introduce the power figure of merit (PFM), to quantify the power that resides within the short pulses as opposed to the background light, or ‘dark energy’ between the pulses. We introduce take advantage of linear sampling using photon counting methods to quantify PFM and demonstrate its use on lasers with PFM ranging from 0.9 to 0.995.
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