Proceedings Article | 28 April 2017
KEYWORDS: Spiral phase plates, Point spread functions, Microscopy, Laser systems engineering, Aberration correction, Detection and tracking algorithms, Modes of laser operation, 3D acquisition, 3D scanning, Optical tweezers
Since a large number of optical systems and devices are based on differently shaped focal intensity distributions (point-spread-functions, PSF), the PSF’s quality is crucial for the application’s performance. E.g., optical tweezers, optical potentials for trapping of ultracold atoms as well as stimulated-emission-depletion (STED) based microscopy and lithography rely on precisely controlled intensity distributions. However, especially in high numerical aperture (NA) systems, such complex laser modes are easily distorted by aberrations leading to performance losses. Although different approaches addressing phase retrieval algorithms have been recently presented[1–3], fast and automated aberration compensation for a broad variety of complex shaped PSFs in high NA systems is still missing.
Here, we report on a Gerchberg-Saxton[4] based algorithm (GSA) for automated aberration correction of arbitrary PSFs, especially for high NA systems. Deviations between the desired target intensity distribution and the three-dimensionally (3D) scanned experimental focal intensity distribution are used to calculate a correction phase pattern. The target phase distribution plus the correction pattern are displayed on a phase-only spatial-light-modulator (SLM). Focused by a high NA objective, experimental 3D scans of several intensity distributions allow for characterization of the algorithms performance: aberrations are reliably identified and compensated within less than 10 iterations.
References
1. B. M. Hanser, M. G. L. Gustafsson, D. A. Agard, and J. W. Sedat, “Phase-retrieved pupil functions in wide-field fluorescence microscopy,” J. of Microscopy 216(1), 32–48 (2004).
2. A. Jesacher, A. Schwaighofer, S. Frhapter, C. Maurer, S. Bernet, and M. Ritsch-Marte, “Wavefront correction of spatial light modulators using an optical vortex image,” Opt. Express 15(9), 5801–5808 (2007).
3. A. Jesacher and M. J. Booth, “Parallel direct laser writing in three dimensions with spatially dependent aberration correction,” Opt. Express 18(20), 21090–21099 (2010).
4. R. W. Gerchberg and W. O. Saxton, “A practical algorithm for the determination of the phase from image and diffraction plane pictures,” Optik 35(2), 237–246 (1972).